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Sample GSM177369 Query DataSets for GSM177369
Status Public on Dec 04, 2007
Title ActCD4TGF, rep2
Sample type RNA
 
Source name Tconv cells activated in vitro with TGFbeta
Organism Mus musculus
Characteristics Genetic background: BDC2.5/NOD
Age: 4-6 wks
Number of mice per chip: 3-6
Tissue: Spleen and LN (cervical, mesenteric)
Sort: B220-, CD11b-, CD8a-, CD69-, CD62Lhi, CD4+, CD25-
Culture: Activation in vitro with anti-CD3/-CD28 beads + IL2 (20 U/ml) + TGFbeta (25ng/ml) for 4 days
Post-culture sort: CD25 hi expressors (top 45%)
Extracted molecule total RNA
Extraction protocol RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
Label Biotin
Label protocol BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
 
Hybridization protocol Per Joslin Diabetes Center microarray core facility.
Scan protocol Per Joslin Diabetes Center microarray core facility.
Description jos3242.CEL
Data processing RMA
 
Submission date Mar 26, 2007
Last update date Aug 28, 2018
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL1261
Series (1)
GSE7460 Contribution of Foxp3 to the Treg signature
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE RMA-unlogged

Data table
ID_REF VALUE
1415670_at 220.5771141
1415671_at 477.7457235
1415672_at 4659.233046
1415673_at 245.8600092
1415674_a_at 423.5652213
1415675_at 240.6040667
1415676_a_at 1620.351246
1415677_at 266.8892177
1415678_at 969.9092384
1415679_at 1953.074295
1415680_at 280.0388119
1415681_at 262.6297973
1415682_at 221.5340133
1415683_at 1109.303045
1415684_at 314.9538285
1415685_at 143.5069683
1415686_at 1697.720056
1415687_a_at 1248.796806
1415688_at 2332.679076
1415689_s_at 404.4479661

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM177369.CEL.gz 3.6 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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