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Sample GSM177370 Query DataSets for GSM177370
Status Public on Dec 04, 2007
Title ActCD4TGF, rep3
Sample type RNA
Source name Tconv cells activated in vitro with TGFbeta
Organism Mus musculus
Characteristics Genetic background: BDC2.5/NOD
Age: 4-6 wks
Number of mice per chip: 3-6
Tissue: Spleen and LN (cervical, mesenteric)
Sort: B220-, CD11b-, CD8a-, CD69-, CD62Lhi, CD4+, CD25-
Culture: Activation in vitro with anti-CD3/-CD28 beads + IL2 (20 U/ml) + TGFbeta (25ng/ml) for 4 days
Post-culture sort: CD25 hi expressors (top 45%)
Extracted molecule total RNA
Extraction protocol RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
Label Biotin
Label protocol BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
Hybridization protocol Per Joslin Diabetes Center microarray core facility.
Scan protocol Per Joslin Diabetes Center microarray core facility.
Description jos3468.CEL
Data processing RMA
Submission date Mar 26, 2007
Last update date Aug 28, 2018
Contact name CBDM Lab
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
Platform ID GPL1261
Series (1)
GSE7460 Contribution of Foxp3 to the Treg signature
Reanalyzed by GSE119085

Data table header descriptions
VALUE RMA-unlogged

Data table
1415670_at 149.3090588
1415671_at 508.4140315
1415672_at 3806.723363
1415673_at 260.5198344
1415674_a_at 363.6237978
1415675_at 362.8990706
1415676_a_at 2064.22936
1415677_at 240.0502033
1415678_at 931.5028664
1415679_at 2548.81764
1415680_at 246.0160255
1415681_at 286.239973
1415682_at 222.9607614
1415683_at 1150.3716
1415684_at 259.0331481
1415685_at 116.0417059
1415686_at 1067.688581
1415687_a_at 1365.491767
1415688_at 1887.834823
1415689_s_at 435.1763079

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.

Supplementary file Size Download File type/resource
GSM177370.CEL.gz 3.5 Mb (ftp)(http) CEL
Processed data included within Sample table
Raw data provided as supplementary file

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