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Sample GSM177375 Query DataSets for GSM177375
Status Public on Dec 04, 2007
Title ActTreg, rep2
Sample type RNA
 
Source name Treg cells activated in vitro
Organism Mus musculus
Characteristics Genetic background: BDC2.5/NOD
Age: 4-6 wks
Number of mice per chip: 3-6
Tissue: Spleen and LN (cervical, mesenteric)
Sort: B220-, CD11b-, CD8a-, CD69-, CD62Lhi, CD4+, CD25+
Culture: Activation in vitro with anti-CD3/-CD28 beads + IL2 (2000 U/ml)for 4 days
Post-culture sort: CD25 hi
Extracted molecule total RNA
Extraction protocol RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
Label Biotin
Label protocol BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
 
Hybridization protocol Per Joslin Diabetes Center microarray core facility.
Scan protocol Per Joslin Diabetes Center microarray core facility.
Description jos3244.CEL
Data processing RMA
 
Submission date Mar 26, 2007
Last update date Aug 28, 2018
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL1261
Series (1)
GSE7460 Contribution of Foxp3 to the Treg signature
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE RMA-unlogged

Data table
ID_REF VALUE
1415670_at 223.275446
1415671_at 380.3886113
1415672_at 4613.702866
1415673_at 302.7698794
1415674_a_at 365.0885806
1415675_at 301.7428433
1415676_a_at 1604.05102
1415677_at 264.7316358
1415678_at 983.0262492
1415679_at 2090.217152
1415680_at 301.9314378
1415681_at 209.9129154
1415682_at 235.4028664
1415683_at 1173.262554
1415684_at 338.6176082
1415685_at 121.0584851
1415686_at 1740.401476
1415687_a_at 1327.606075
1415688_at 2269.251886
1415689_s_at 389.6860733

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM177375.CEL.gz 3.6 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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