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Sample GSM177377 Query DataSets for GSM177377
Status Public on Dec 04, 2007
Title ActTregTGF, rep2
Sample type RNA
 
Source name Treg cells activated in vitro with TGFbeta
Organism Mus musculus
Characteristics Genetic background: BDC2.5/NOD
Age: 4-6 wks
Number of mice per chip: 3-6
Tissue: Spleen and LN (cervical, mesenteric)
Sort: B220-, CD11b-, CD8a-, CD69-, CD62Lhi, CD4+, CD25+
Culture: Activation in vitro with anti-CD3/-CD28 beads + IL2 (2000 U/ml) + TGFbeta (25ng/ml) for 4 days
Post-culture sort: CD25 hi
Extracted molecule total RNA
Extraction protocol RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
Label Biotin
Label protocol BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
 
Hybridization protocol Per Joslin Diabetes Center microarray core facility.
Scan protocol Per Joslin Diabetes Center microarray core facility.
Description jos3245.CEL
Data processing RMA
 
Submission date Mar 26, 2007
Last update date Aug 28, 2018
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL1261
Series (1)
GSE7460 Contribution of Foxp3 to the Treg signature
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE RMA-unlogged

Data table
ID_REF VALUE
1415670_at 183.3969265
1415671_at 452.7342265
1415672_at 4526.365928
1415673_at 378.710712
1415674_a_at 397.9649102
1415675_at 384.9706109
1415676_a_at 1735.936809
1415677_at 270.0023401
1415678_at 1136.218377
1415679_at 2632.964408
1415680_at 285.0897281
1415681_at 244.4002909
1415682_at 219.8589869
1415683_at 1137.183738
1415684_at 394.6394172
1415685_at 144.0435597
1415686_at 1726.288581
1415687_a_at 1160.63098
1415688_at 2422.761135
1415689_s_at 433.6536632

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM177377.CEL.gz 3.4 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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