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GEO help: Mouse over screen elements for information. |
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Status |
Public on Dec 04, 2007 |
Title |
ActTregTGF, rep2 |
Sample type |
RNA |
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Source name |
Treg cells activated in vitro with TGFbeta
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Organism |
Mus musculus |
Characteristics |
Genetic background: BDC2.5/NOD Age: 4-6 wks Number of mice per chip: 3-6 Tissue: Spleen and LN (cervical, mesenteric) Sort: B220-, CD11b-, CD8a-, CD69-, CD62Lhi, CD4+, CD25+ Culture: Activation in vitro with anti-CD3/-CD28 beads + IL2 (2000 U/ml) + TGFbeta (25ng/ml) for 4 days Post-culture sort: CD25 hi
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Extracted molecule |
total RNA |
Extraction protocol |
RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
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Label |
Biotin
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Label protocol |
BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
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Hybridization protocol |
Per Joslin Diabetes Center microarray core facility.
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Scan protocol |
Per Joslin Diabetes Center microarray core facility.
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Description |
jos3245.CEL
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Data processing |
RMA
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Submission date |
Mar 26, 2007 |
Last update date |
Aug 28, 2018 |
Contact name |
CBDM Lab |
E-mail(s) |
cbdm@hms.harvard.edu
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Phone |
617-432-7747
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Organization name |
Harvard Medical School
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Department |
Microbiology and Immunobiology
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Lab |
CBDM
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Street address |
77 Avenue Louis Pasteur
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City |
Boston |
State/province |
MA |
ZIP/Postal code |
02215 |
Country |
USA |
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Platform ID |
GPL1261 |
Series (1) |
GSE7460 |
Contribution of Foxp3 to the Treg signature |
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Relations |
Reanalyzed by |
GSE119085 |
Data table header descriptions |
ID_REF |
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VALUE |
RMA-unlogged |
Data table |
ID_REF |
VALUE |
1415670_at |
183.3969265 |
1415671_at |
452.7342265 |
1415672_at |
4526.365928 |
1415673_at |
378.710712 |
1415674_a_at |
397.9649102 |
1415675_at |
384.9706109 |
1415676_a_at |
1735.936809 |
1415677_at |
270.0023401 |
1415678_at |
1136.218377 |
1415679_at |
2632.964408 |
1415680_at |
285.0897281 |
1415681_at |
244.4002909 |
1415682_at |
219.8589869 |
1415683_at |
1137.183738 |
1415684_at |
394.6394172 |
1415685_at |
144.0435597 |
1415686_at |
1726.288581 |
1415687_a_at |
1160.63098 |
1415688_at |
2422.761135 |
1415689_s_at |
433.6536632 |
Total number of rows: 45101
Table truncated, full table size 1026 Kbytes.
Supplementary file |
Size |
Download |
File type/resource |
GSM177377.CEL.gz |
3.4 Mb |
(ftp)(http) |
CEL |
Processed data included within Sample table |
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