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Sample GSM177378 Query DataSets for GSM177378
Status Public on Dec 04, 2007
Title ActTregTGF, rep3
Sample type RNA
 
Source name Treg cells activated in vitro with TGFbeta
Organism Mus musculus
Characteristics Genetic background: BDC2.5/NOD
Age: 4-6 wks
Number of mice per chip: 3-6
Tissue: Spleen and LN (cervical, mesenteric)
Sort: B220-, CD11b-, CD8a-, CD69-, CD62Lhi, CD4+, CD25+
Culture: Activation in vitro with anti-CD3/-CD28 beads + IL2 (2000 U/ml) + TGFbeta (25ng/ml) for 4 days
Post-culture sort: CD25 hi
Extracted molecule total RNA
Extraction protocol RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
Label Biotin
Label protocol BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
 
Hybridization protocol Per Joslin Diabetes Center microarray core facility.
Scan protocol Per Joslin Diabetes Center microarray core facility.
Description jos3317.CEL
Data processing RMA
 
Submission date Mar 26, 2007
Last update date Aug 28, 2018
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL1261
Series (1)
GSE7460 Contribution of Foxp3 to the Treg signature
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE RMA-unlogged

Data table
ID_REF VALUE
1415670_at 153.6731136
1415671_at 362.9963137
1415672_at 4102.002534
1415673_at 264.7885806
1415674_a_at 358.6001346
1415675_at 360.4765865
1415676_a_at 1934.480241
1415677_at 233.0017848
1415678_at 748.6326523
1415679_at 2405.945068
1415680_at 279.7790935
1415681_at 243.1988098
1415682_at 237.6590468
1415683_at 1216.489575
1415684_at 291.7741251
1415685_at 140.3982273
1415686_at 1241.075225
1415687_a_at 1370.968713
1415688_at 2054.413177
1415689_s_at 334.1542955

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM177378.CEL.gz 3.4 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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