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Sample GSM177382 Query DataSets for GSM177382
Status Public on Dec 04, 2007
Title wtActCD4, rep2
Sample type RNA
 
Source name Tconv cells activated in vitro
Organism Mus musculus
Characteristics Genetic background: BDC2.5/NOD.Foxp3+
Age: 10 day old
Number of mice per chip: 3-6
Tissue: Spleen
Sort: enriched with anti-CD4 MACS beads
Culture: Activation in vitro with anti-CD3/-CD28 beads + IL2 (20 U/ml) for 4 days
Post-culture sort: CD4+, CD25 hi (top 45%)
Extracted molecule total RNA
Extraction protocol RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
Label Biotin
Label protocol BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
 
Hybridization protocol Per Joslin Diabetes Center microarray core facility.
Scan protocol Per Joslin Diabetes Center microarray core facility.
Description jos3291.CEL
Data processing RMA
 
Submission date Mar 26, 2007
Last update date Aug 28, 2018
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL1261
Series (1)
GSE7460 Contribution of Foxp3 to the Treg signature
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE RMA-unlogged

Data table
ID_REF VALUE
1415670_at 226.8987818
1415671_at 387.9573724
1415672_at 4941.966989
1415673_at 422.6390911
1415674_a_at 391.0171521
1415675_at 224.1799518
1415676_a_at 1670.078888
1415677_at 273.9292171
1415678_at 876.2669771
1415679_at 1645.55428
1415680_at 457.4540192
1415681_at 270.839875
1415682_at 307.0849487
1415683_at 1508.963505
1415684_at 340.3907627
1415685_at 130.5335901
1415686_at 1581.874295
1415687_a_at 1042.561302
1415688_at 2807.841125
1415689_s_at 407.1875525

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM177382.CEL.gz 3.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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