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Sample GSM177390 Query DataSets for GSM177390
Status Public on Dec 04, 2007
Title wtActCD4TGF, rep3
Sample type RNA
 
Source name Tconv cells activated in vitro with TGFbeta
Organism Mus musculus
Characteristics Genetic background: BDC2.5/NOD.Foxp3+
Age: 10 days
Number of mice per chip: 3-6
Tissue: Spleen
Sort: Enrichment with anti-CD4 MACS beads
Culture: Activation in vitro with anti-CD3/-CD28 beads + IL2 (20 U/ml) + TGFbeta (25ng/ml) for 4 days
Post-culture sort: CD4+, CD25 hi (top 45%)
Extracted molecule total RNA
Extraction protocol RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
Label Biotin
Label protocol BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
 
Hybridization protocol Per Joslin Diabetes Center microarray core facility.
Scan protocol Per Joslin Diabetes Center microarray core facility.
Description jos3370.CEL
Data processing RMA
 
Submission date Mar 26, 2007
Last update date Aug 28, 2018
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL1261
Series (1)
GSE7460 Contribution of Foxp3 to the Treg signature
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE RMA-unlogged

Data table
ID_REF VALUE
1415670_at 168.1858979
1415671_at 412.9603207
1415672_at 4308.720552
1415673_at 432.2885806
1415674_a_at 378.8815705
1415675_at 397.2643092
1415676_a_at 2345.898062
1415677_at 264.3414112
1415678_at 793.4635368
1415679_at 2758.571861
1415680_at 298.4925762
1415681_at 308.4355669
1415682_at 239.4237979
1415683_at 1523.548467
1415684_at 268.1476963
1415685_at 136.9091735
1415686_at 1219.917152
1415687_a_at 1173.413884
1415688_at 1846.124662
1415689_s_at 358.7045531

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM177390.CEL.gz 3.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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