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Sample GSM177391 Query DataSets for GSM177391
Status Public on Dec 04, 2007
Title sfActCD4, rep2
Sample type RNA
Source name Tconv cells activated in vitro
Organism Mus musculus
Characteristics Genetic background: BDC2.5/NOD.Foxp3sf
Age: 10 days
Number of mice per chip: 3-6
Tissue: Spleen
Sort: Enrichment with anti-CD4 MACS beads
Culture: Activation in vitro with anti-CD3/-CD28 beads + IL2 (20 U/ml) for 4 days
Post-culture sort: CD4+, CD25 hi (top 45%)
Extracted molecule total RNA
Extraction protocol RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
Label Biotin
Label protocol BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
Hybridization protocol Per Joslin Diabetes Center microarray core facility.
Scan protocol Per Joslin Diabetes Center microarray core facility.
Description jos3289.CEL
Data processing RMA
Submission date Mar 26, 2007
Last update date Aug 28, 2018
Contact name CBDM Lab
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
Platform ID GPL1261
Series (1)
GSE7460 Contribution of Foxp3 to the Treg signature
Reanalyzed by GSE119085

Data table header descriptions
VALUE RMA-unlogged

Data table
1415670_at 211.4334602
1415671_at 502.7013587
1415672_at 4881.107481
1415673_at 395.2603581
1415674_a_at 362.6107355
1415675_at 260.1885806
1415676_a_at 1433.874295
1415677_at 302.7580261
1415678_at 882.6145662
1415679_at 2091.256854
1415680_at 374.4599203
1415681_at 216.3878514
1415682_at 204.2933532
1415683_at 1474.755439
1415684_at 311.9081298
1415685_at 150.7314379
1415686_at 1461.134879
1415687_a_at 1453.06527
1415688_at 2521.785296
1415689_s_at 483.452044

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.

Supplementary file Size Download File type/resource
GSM177391.CEL.gz 3.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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