|
| Status |
Public on Jul 06, 2015 |
| Title |
caudal parts of the corpus callosum-12 weeks-Wild_Type-4 |
| Sample type |
RNA |
| |
|
| Source name |
caudal parts of the corpus callosum
|
| Organism |
Mus musculus |
| Characteristics |
diet: mice standard diet containing 0.2% powdered CPZ (oxalic bis(cyclohexylidenehydrazide))
strain: C57BL/6
Stage: 12 weeks
genotype/variation: Wild_Type
|
| Treatment protocol |
Six to tweelve-week-old TREM2-/- and WT mice were fed a standard diet (Harlan) containing 0.2% CPZ [finely powdered oxalic bis(cyclohexylidenehydrazide); Sigma-Aldrich] for 4, 6 or 12 weeks.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy mini kit (Qiagen) according to the manufacturer’s protocol
|
| Label |
biotin
|
| Label protocol |
NuGen WT- Ovation Pico RNA Amplification
|
| |
|
| Hybridization protocol |
Following fragmentation with NuGen Encore Biotin Module, 2.5 ug of cDNA were hybridized into the GeneChip Hybridization Oven 640 for 18 h at 45 °C on Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
|
| Scan protocol |
Affymetrix GeneChip 7G 3000 Scanner
|
| Description |
Gene expression data from Twelve week-old WT mice fed a standard diet containing 0.2% CPZ
|
| Data processing |
Command Console (Affymetrix, Inc),Robust Multichip Analysis package ‘oligo’ version 1.30.0 target Level of summarization=core
|
| |
|
| Submission date |
Jul 02, 2015 |
| Last update date |
Jul 06, 2015 |
| Contact name |
Danilo Licastro |
| E-mail(s) |
danilo.licastro@cbm.fvg.it
|
| Phone |
+390403757720
|
| Organization name |
CBM
|
| Department |
Genomica Funzionale
|
| Lab |
T62
|
| Street address |
SS 14, Km. 163,5
|
| City |
Trieste |
| ZIP/Postal code |
34012 |
| Country |
Italy |
| |
|
| Platform ID |
GPL10740 |
| Series (1) |
| GSE70475 |
TREM2 regulates microglial cell activation in response to demyelination in vivo |
|
