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Sample GSM178673 Query DataSets for GSM178673
Status Public on Mar 31, 2007
Title Burn Spleen 2 hr rep 2
Sample type RNA
 
Source name mouse splenocytes
Organism Mus musculus
Characteristics Male C57BL/6J
Treatment protocol Leukocyte isolation from cardiac puncture followed by RBC lysis with proprietary buffer, followed by suspension in RLT buffer ; Splenocytes in Dulbeco's PBS followed by the same RBC lysis RLT suspension process.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using the manufacturer’s recommended protocol for RNeasy (Qiagen, Valencia, CA).
Label biotin
Label protocol 1-2 μg of total RNA was used to make single-stranded antisense cDNA using the NuGEN Technologies (San Carlo, CA) Ovation™ Biotin System in accordance with the manufacturer’s directions.
 
Hybridization protocol Labeled targets were hybridized to Affymetrix (Santa Clara, CA) Mouse Genome 430 2.0 Array GeneChip™ microarrays for 16 hours at 45° C. The arrays were washed and scanned according to Affymetrix standard protocols
Scan protocol Scanned according to Affymetrix standard protocols.
Description trauma hemorrage injury model
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0/GCOS 1.4 ) using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Mar 29, 2007
Last update date Aug 28, 2018
Contact name Wenzhong Xiao, Inflammation & the Host Response to Injury
E-mail(s) data_curator@gluegrant.org
Phone 617 726 0082
Organization name Massachusetts General Hospital
Department Department of Surgery
Lab Burn & Trauma Research
Street address 55 Fruit Street, GRV 1302
City Boston
State/province MA
ZIP/Postal code 02114
Country USA
 
Platform ID GPL1261
Series (1)
GSE7404 Comparison of Longitudinal Leukocyte Gene Expression after Burn Injury or Trauma Hemorrhage in Mice
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE MAS 5.0 Signal Intensity
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 4191.46 P 4.42873e-05
AFFX-BioB-M_at 7904.28 P 4.42873e-05
AFFX-BioB-3_at 6365.99 P 4.42873e-05
AFFX-BioC-5_at 13330.5 P 4.42873e-05
AFFX-BioC-3_at 9095 P 4.42873e-05
AFFX-BioDn-5_at 21778 P 4.42873e-05
AFFX-BioDn-3_at 31384.4 P 4.42873e-05
AFFX-CreX-5_at 59775.6 P 5.16732e-05
AFFX-CreX-3_at 61981.2 P 4.42873e-05
AFFX-DapX-5_at 26.2918 A 0.617411
AFFX-DapX-M_at 28.2482 A 0.645547
AFFX-DapX-3_at 6.30804 A 0.921998
AFFX-LysX-5_at 40.6717 A 0.354441
AFFX-LysX-M_at 10.5019 A 0.904333
AFFX-LysX-3_at 35.0425 A 0.544587
AFFX-PheX-5_at 10.2307 A 0.963431
AFFX-PheX-M_at 57.9297 A 0.250777
AFFX-PheX-3_at 12.1132 A 0.382599
AFFX-ThrX-5_at 4.89378 A 0.897855
AFFX-ThrX-M_at 50.9316 A 0.340648

Total number of rows: 45101

Table truncated, full table size 1377 Kbytes.




Supplementary file Size Download File type/resource
GSM178673.CEL.gz 5.8 Mb (ftp)(http) CEL
GSM178673.CHP.gz 243.4 Kb (ftp)(http) CHP
GSM178673.EXP.gz 350 b (ftp)(http) EXP
Processed data provided as supplementary file
Processed data included within Sample table

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