|
Status |
Public on Mar 31, 2007 |
Title |
Burn Sham Spleen 1 day rep 2 |
Sample type |
RNA |
|
|
Source name |
mouse splenocytes
|
Organism |
Mus musculus |
Characteristics |
Male C57BL/6J
|
Treatment protocol |
Leukocyte isolation from cardiac puncture followed by RBC lysis with proprietary buffer, followed by suspension in RLT buffer ; Splenocytes in Dulbeco's PBS followed by the same RBC lysis RLT suspension process.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using the manufacturer’s recommended protocol for RNeasy (Qiagen, Valencia, CA).
|
Label |
biotin
|
Label protocol |
1-2 μg of total RNA was used to make single-stranded antisense cDNA using the NuGEN Technologies (San Carlo, CA) Ovation™ Biotin System in accordance with the manufacturer’s directions.
|
|
|
Hybridization protocol |
Labeled targets were hybridized to Affymetrix (Santa Clara, CA) Mouse Genome 430 2.0 Array GeneChip™ microarrays for 16 hours at 45° C. The arrays were washed and scanned according to Affymetrix standard protocols
|
Scan protocol |
Scanned according to Affymetrix standard protocols.
|
Description |
trauma hemorrage injury model
|
Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0/GCOS 1.4 ) using Affymetrix default analysis settings and global scaling as normalization method.
|
|
|
Submission date |
Mar 29, 2007 |
Last update date |
Aug 28, 2018 |
Contact name |
Wenzhong Xiao, Inflammation & the Host Response to Injury |
E-mail(s) |
data_curator@gluegrant.org
|
Phone |
617 726 0082
|
Organization name |
Massachusetts General Hospital
|
Department |
Department of Surgery
|
Lab |
Burn & Trauma Research
|
Street address |
55 Fruit Street, GRV 1302
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02114 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE7404 |
Comparison of Longitudinal Leukocyte Gene Expression after Burn Injury or Trauma Hemorrhage in Mice |
|
Relations |
Reanalyzed by |
GSE119085 |