|
| Status |
Public on Mar 31, 2007 |
| Title |
Burn Sham Spleen 3 day rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
mouse splenocytes
|
| Organism |
Mus musculus |
| Characteristics |
Male C57BL/6J
|
| Treatment protocol |
Leukocyte isolation from cardiac puncture followed by RBC lysis with proprietary buffer, followed by suspension in RLT buffer ; Splenocytes in Dulbeco's PBS followed by the same RBC lysis RLT suspension process.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the manufacturer’s recommended protocol for RNeasy (Qiagen, Valencia, CA).
|
| Label |
biotin
|
| Label protocol |
1-2 μg of total RNA was used to make single-stranded antisense cDNA using the NuGEN Technologies (San Carlo, CA) Ovation™ Biotin System in accordance with the manufacturer’s directions.
|
| |
|
| Hybridization protocol |
Labeled targets were hybridized to Affymetrix (Santa Clara, CA) Mouse Genome 430 2.0 Array GeneChip™ microarrays for 16 hours at 45° C. The arrays were washed and scanned according to Affymetrix standard protocols
|
| Scan protocol |
Scanned according to Affymetrix standard protocols.
|
| Description |
trauma hemorrage injury model
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0/GCOS 1.4 ) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Mar 29, 2007 |
| Last update date |
Mar 18, 2013 |
| Contact name |
Wenzhong Xiao, Inflammation & the Host Response to Injury |
| E-mail(s) |
data_curator@gluegrant.org
|
| Phone |
617 726 0082
|
| Organization name |
Massachusetts General Hospital
|
| Department |
Department of Surgery
|
| Lab |
Burn & Trauma Research
|
| Street address |
55 Fruit Street, GRV 1302
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE7404 |
Comparison of Longitudinal Leukocyte Gene Expression after Burn Injury or Trauma Hemorrhage in Mice |
|
