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Sample GSM178733 Query DataSets for GSM178733
Status Public on Mar 31, 2007
Title Trauma Hemorrhage Sham Spleen 7 day rep 2
Sample type RNA
 
Source name mouse splenocytes
Organism Mus musculus
Characteristics Male C57BL/6J
Treatment protocol Leukocyte isolation from cardiac puncture followed by RBC lysis with proprietary buffer, followed by suspension in RLT buffer ; Splenocytes in Dulbeco's PBS followed by the same RBC lysis RLT suspension process.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using the manufacturer’s recommended protocol for RNeasy (Qiagen, Valencia, CA).
Label biotin
Label protocol 1-2 μg of total RNA was used to make single-stranded antisense cDNA using the NuGEN Technologies (San Carlo, CA) Ovation™ Biotin System in accordance with the manufacturer’s directions.
 
Hybridization protocol Labeled targets were hybridized to Affymetrix (Santa Clara, CA) Mouse Genome 430 2.0 Array GeneChip™ microarrays for 16 hours at 45° C. The arrays were washed and scanned according to Affymetrix standard protocols
Scan protocol Scanned according to Affymetrix standard protocols.
Description trauma hemorrage injury model
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0/GCOS 1.4 ) using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Mar 29, 2007
Last update date Aug 28, 2018
Contact name Wenzhong Xiao, Inflammation & the Host Response to Injury
E-mail(s) data_curator@gluegrant.org
Phone 617 726 0082
Organization name Massachusetts General Hospital
Department Department of Surgery
Lab Burn & Trauma Research
Street address 55 Fruit Street, GRV 1302
City Boston
State/province MA
ZIP/Postal code 02114
Country USA
 
Platform ID GPL1261
Series (1)
GSE7404 Comparison of Longitudinal Leukocyte Gene Expression after Burn Injury or Trauma Hemorrhage in Mice
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE MAS 5.0 Signal Intensity
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 11369.8 P 4.42873e-05
AFFX-BioB-M_at 20691 P 4.42873e-05
AFFX-BioB-3_at 16477 P 4.42873e-05
AFFX-BioC-5_at 34474.9 P 4.42873e-05
AFFX-BioC-3_at 27518.7 P 4.42873e-05
AFFX-BioDn-5_at 59884.3 P 4.42873e-05
AFFX-BioDn-3_at 83531.6 P 4.42873e-05
AFFX-CreX-5_at 166751 P 5.16732e-05
AFFX-CreX-3_at 179407 P 4.42873e-05
AFFX-DapX-5_at 23.3395 A 0.603116
AFFX-DapX-M_at 5.52656 A 0.963456
AFFX-DapX-3_at 108.494 P 0.0464104
AFFX-LysX-5_at 5.75748 A 0.672921
AFFX-LysX-M_at 7.81944 A 0.904352
AFFX-LysX-3_at 15.9432 A 0.876489
AFFX-PheX-5_at 14.4154 A 0.737173
AFFX-PheX-M_at 42.0069 A 0.57405
AFFX-PheX-3_at 26.1357 A 0.588627
AFFX-ThrX-5_at 21.4168 A 0.897855
AFFX-ThrX-M_at 56.2824 A 0.470241

Total number of rows: 45101

Table truncated, full table size 1375 Kbytes.




Supplementary file Size Download File type/resource
GSM178733.CEL.gz 5.4 Mb (ftp)(http) CEL
GSM178733.CHP.gz 248.4 Kb (ftp)(http) CHP
GSM178733.EXP.gz 351 b (ftp)(http) EXP
Processed data provided as supplementary file
Processed data included within Sample table

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