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Sample GSM180344 Query DataSets for GSM180344
Status Public on Jul 27, 2007
Title E2F4 in Jurkat extended promoter array 2 rep2
Sample type genomic
 
Channel 1
Source name Jurkat cells immunoprecipitated
Organism Homo sapiens
Characteristics anti E2F4 antibody; chromatin immunoprecipitation
Extracted molecule genomic DNA
Extraction protocol Phenol Chloroform extraction
Label Cy5
Label protocol Labeled using random primed klenow based extension
 
Channel 2
Source name Jurkat cells Input DNA (total)
Organism Homo sapiens
Characteristics Jurkat cells Input DNA
Extracted molecule genomic DNA
Extraction protocol Phenol Chloroform extraction
Label Cy3
Label protocol Labeled using random primed klenow based extension
 
 
Hybridization protocol Hybridization of 2 micrograms of each labeled DNA for 40 hrs at 65 degrees using Agilent ACGH/ChIP-chip hybridization and wash kit
Scan protocol Hybridized microarrays were scanned using an Agilent G2565BA microarray scanner.
Description Chromatin IP signal
Data processing Raw image files were processed with Agilent Feature Extraction software (v. 8.5).
 
Submission date Apr 03, 2007
Last update date Jul 27, 2007
Contact name Peter Hollenhorst
E-mail(s) pchollen@iupui.edu
Organization name Indiana University
Street address 1001 E Third St, Jordan Hall 104
City Bloomington
State/province IN
ZIP/Postal code 47405
Country USA
 
Platform ID GPL4125
Series (1)
GSE7449 Genome-wide analysis reveals determinants of redundant and specific binding within a transcription factor family

Data table header descriptions
ID_REF
VALUE Log10 ratio of red (IP) to green (WCE) signals
gProcessedSignal Whole cell extract (WCE or Input) mean signal minus background
rProcessedSignal IP mean signal minus background

Data table
ID_REF VALUE gProcessedSignal rProcessedSignal
1 -1.242920007e-001 2.082311e+004 1.564061e+004
2 0.000000000e+000 7.813867e+001 3.811017e+001
3 0.000000000e+000 7.807448e+001 3.808616e+001
4 -1.836257756e-001 1.791165e+003 1.173573e+003
5 -4.667290677e-002 2.492214e+002 2.238270e+002
6 -7.880166036e-001 4.419880e+002 7.201018e+001
7 -2.872955508e-001 2.258967e+003 1.165774e+003
8 -1.946449886e-002 1.085804e+003 1.038214e+003
9 -1.093665897e-001 6.444381e+002 5.009733e+002
10 2.098142438e-001 5.946144e+002 9.639392e+002
11 -7.823665175e-002 2.796883e+002 2.335811e+002
12 -2.967748208e-002 1.693091e+003 1.581258e+003
13 4.908727312e-001 8.059738e+002 2.495707e+003
14 4.701812895e-001 3.810165e+002 1.124929e+003
15 -1.441040735e-001 6.785529e+002 4.869447e+002
16 -2.023800449e-002 1.103308e+003 1.053074e+003
17 4.220475495e-001 1.025423e+003 2.709885e+003
18 -6.756852535e-002 7.172267e+002 6.138863e+002
19 1.906991681e-001 3.650234e+002 5.662652e+002
20 -2.151512846e-001 5.799925e+002 3.534037e+002

Total number of rows: 243485

Table truncated, full table size 12375 Kbytes.




Supplementary file Size Download File type/resource
GSM180344.txt.gz 63.9 Mb (ftp)(http) TXT
Processed data included within Sample table

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