|
Status |
Public on Jul 27, 2007 |
Title |
E2F4 in Jurkat extended promoter array 2 rep2 |
Sample type |
genomic |
|
|
Channel 1 |
Source name |
Jurkat cells immunoprecipitated
|
Organism |
Homo sapiens |
Characteristics |
anti E2F4 antibody; chromatin immunoprecipitation
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Phenol Chloroform extraction
|
Label |
Cy5
|
Label protocol |
Labeled using random primed klenow based extension
|
|
|
Channel 2 |
Source name |
Jurkat cells Input DNA (total)
|
Organism |
Homo sapiens |
Characteristics |
Jurkat cells Input DNA
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Phenol Chloroform extraction
|
Label |
Cy3
|
Label protocol |
Labeled using random primed klenow based extension
|
|
|
|
Hybridization protocol |
Hybridization of 2 micrograms of each labeled DNA for 40 hrs at 65 degrees using Agilent ACGH/ChIP-chip hybridization and wash kit
|
Scan protocol |
Hybridized microarrays were scanned using an Agilent G2565BA microarray scanner.
|
Description |
Chromatin IP signal
|
Data processing |
Raw image files were processed with Agilent Feature Extraction software (v. 8.5).
|
|
|
Submission date |
Apr 03, 2007 |
Last update date |
Jul 27, 2007 |
Contact name |
Peter Hollenhorst |
E-mail(s) |
pchollen@iupui.edu
|
Organization name |
Indiana University
|
Street address |
1001 E Third St, Jordan Hall 104
|
City |
Bloomington |
State/province |
IN |
ZIP/Postal code |
47405 |
Country |
USA |
|
|
Platform ID |
GPL4125 |
Series (1) |
GSE7449 |
Genome-wide analysis reveals determinants of redundant and specific binding within a transcription factor family |
|