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Sample GSM180478 Query DataSets for GSM180478
Status Public on Dec 04, 2007
Title sfActCD4TGF, rep2
Sample type RNA
 
Source name Tconv cells activated in vitro with TGFbeta
Organism Mus musculus
Characteristics Genetic background: BDC2.5/NOD.Foxp3sf
Age: 10 days
Number of mice per chip: 3-6
Tissue: Spleen
Sort: Enrichment with anti-CD4 MACS beads
Culture: Activation in vitro with anti-CD3/-CD28 beads + IL2 (20 U/ml) + TGFbeta (25ng/ml) for 4 days
Post-culture sort: CD4+, CD25 hi expressers (top 45%)
Extracted molecule total RNA
Extraction protocol RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
Label Biotin
Label protocol BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
 
Hybridization protocol Per Joslin Diabetes Center microarray core facility.
Scan protocol Per Joslin Diabetes Center microarray core facility.
Description jos3290.CEL
Data processing RMA
 
Submission date Apr 04, 2007
Last update date Aug 28, 2018
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL1261
Series (1)
GSE7460 Contribution of Foxp3 to the Treg signature
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE RMA-unlogged

Data table
ID_REF VALUE
1415670_at 226.6934157
1415671_at 494.1939415
1415672_at 5244.396487
1415673_at 413.0314378
1415674_a_at 422.8600092
1415675_at 270.1171521
1415676_a_at 1554.813555
1415677_at 285.2162285
1415678_at 732.4231171
1415679_at 1700.392061
1415680_at 353.0638838
1415681_at 289.2683742
1415682_at 201.3160293
1415683_at 1666.130641
1415684_at 324.4189699
1415685_at 150.2669164
1415686_at 1433.818723
1415687_a_at 1258.292301
1415688_at 2137.071701
1415689_s_at 360.5988397

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM180478.CEL.gz 3.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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