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Sample GSM180486 Query DataSets for GSM180486
Status Public on Dec 04, 2007
Title sfActCD4TGF, rep3
Sample type RNA
 
Source name Tconv cells activated in vitro with TGFbeta
Organism Mus musculus
Characteristics Genetic background: BDC2.5/NOD.Foxp3sf
Age: 10 days
Number of mice per chip: 3-6
Tissue: Spleen
Sort: Enrichment with anti-CD4 MACS beads
Culture: Activation in vitro with anti-CD3/-CD28 beads + IL2 (20 U/ml) + TGFbeta (25ng/ml) for 4 days
Post-culture sort: CD4+, CD25 hi expressers (top 45%)
Extracted molecule total RNA
Extraction protocol RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
Label Biotin
Label protocol BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
 
Hybridization protocol Per Joslin Diabetes Center microarray core facility.
Scan protocol Per Joslin Diabetes Center microarray core facility.
Description jos3367.CEL
Data processing RMA
 
Submission date Apr 04, 2007
Last update date Aug 28, 2018
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL1261
Series (1)
GSE7460 Contribution of Foxp3 to the Treg signature
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE RMA-unlogged

Data table
ID_REF VALUE
1415670_at 221.593314
1415671_at 412.6191506
1415672_at 4905.822672
1415673_at 567.0683169
1415674_a_at 431.1170722
1415675_at 406.7367265
1415676_a_at 2714.19784
1415677_at 254.1728304
1415678_at 702.8143075
1415679_at 2456.14364
1415680_at 320.5156551
1415681_at 363.6494364
1415682_at 275.7798751
1415683_at 1745.448583
1415684_at 336.9337554
1415685_at 123.3491797
1415686_at 1316.543016
1415687_a_at 1134.190776
1415688_at 1745.03004
1415689_s_at 301.3618404

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM180486.CEL.gz 3.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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