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Sample GSM180489 Query DataSets for GSM180489
Status Public on Dec 04, 2007
Title wtActCD4, rep3
Sample type RNA
 
Source name Tconv cells activated in vitro
Organism Mus musculus
Characteristics Genetic background: BDC2.5/NOD.Foxp3+
Age: 10 day old
Number of mice per chip: 3-6
Tissue: Spleen
Sort: enriched with anti-CD4 MACS beads
Culture: Activation in vitro with anti-CD3/-CD28 beads + IL2 (20 U/ml) for 4 days
Post-culture sort: CD4+, CD25 hi (top 45%)
Extracted molecule total RNA
Extraction protocol RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
Label Biotin
Label protocol BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
 
Hybridization protocol Per Joslin Diabetes Center microarray core facility.
Scan protocol Per Joslin Diabetes Center microarray core facility.
Description jos3369.CEL
Data processing RMA
 
Submission date Apr 04, 2007
Last update date Aug 28, 2018
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL1261
Series (1)
GSE7460 Contribution of Foxp3 to the Treg signature
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE RMA-unlogged

Data table
ID_REF VALUE
1415670_at 240.1310052
1415671_at 360.8742949
1415672_at 3974.802866
1415673_at 694.0624918
1415674_a_at 592.9728005
1415675_at 382.7393448
1415676_a_at 2733.574295
1415677_at 249.5198918
1415678_at 776.7885806
1415679_at 2121.360009
1415680_at 412.2563636
1415681_at 426.9495814
1415682_at 328.9894522
1415683_at 1954.10059
1415684_at 325.8084452
1415685_at 161.6097835
1415686_at 1443.423771
1415687_a_at 796.0969603
1415688_at 1814.371464
1415689_s_at 280.3792828

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM180489.CEL.gz 3.4 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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