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Sample GSM180492 Query DataSets for GSM180492
Status Public on Dec 04, 2007
Title ActCD8, rep2
Sample type RNA
 
Source name CD8 T cells activated in vitro
Organism Mus musculus
Characteristics Genetic background: BDC2.5/NOD
Age: 4-6 wks
Number of mice per chip: 3-6
Tissue: Spleen and LN (cervical, mesenteric)
Sort: B220-, CD11b-, CD4-, CD69-, CD25-, CD62Lhi, CD8a+,
Culture: Activation in vitro with anti-CD3/-CD28 beads + IL2 (20 U/ml) for 4 days
Post-culture sort: CD8a hi expressers
Extracted molecule total RNA
Extraction protocol RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
Label Biotin
Label protocol BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
 
Hybridization protocol Per Joslin Diabetes Center microarray core facility.
Scan protocol Per Joslin Diabetes Center microarray core facility.
Description jos3469.CEL
Data processing RMA
 
Submission date Apr 04, 2007
Last update date Aug 28, 2018
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL1261
Series (1)
GSE7460 Contribution of Foxp3 to the Treg signature
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE RMA-unlogged

Data table
ID_REF VALUE
1415670_at 144.8603503
1415671_at 370.4860877
1415672_at 3773.179522
1415673_at 344.7314378
1415674_a_at 325.507685
1415675_at 298.4055127
1415676_a_at 1727.846782
1415677_at 261.8171521
1415678_at 772.220452
1415679_at 2251.464185
1415680_at 272.4028664
1415681_at 237.4913766
1415682_at 204.3631351
1415683_at 907.1231673
1415684_at 235.4314378
1415685_at 130.6461705
1415686_at 745.3607146
1415687_a_at 1818.19958
1415688_at 1729.688828
1415689_s_at 447.2703446

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM180492.CEL.gz 2.8 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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