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Sample GSM180493 Query DataSets for GSM180493
Status Public on Dec 04, 2007
Title ActCD8, rep3
Sample type RNA
 
Source name CD8 T cells activated in vitro
Organism Mus musculus
Characteristics Genetic background: BDC2.5/NOD
Age: 4-6 wks
Number of mice per chip: 3-6
Tissue: Spleen and LN (cervical, mesenteric)
Sort: B220-, CD11b-, CD4-, CD69-, CD25-, CD62Lhi, CD8a+,
Culture: Activation in vitro with anti-CD3/-CD28 beads + IL2 (20 U/ml) for 4 days
Post-culture sort: CD8a hi expressers
Extracted molecule total RNA
Extraction protocol RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
Label Biotin
Label protocol BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
 
Hybridization protocol Per Joslin Diabetes Center microarray core facility.
Scan protocol Per Joslin Diabetes Center microarray core facility.
Description jos3471.CEL
Data processing RMA
 
Submission date Apr 04, 2007
Last update date Aug 28, 2018
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL1261
Series (1)
GSE7460 Contribution of Foxp3 to the Treg signature
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE RMA-unlogged

Data table
ID_REF VALUE
1415670_at 148.0937886
1415671_at 411.982834
1415672_at 4190.866742
1415673_at 298.0749357
1415674_a_at 333.9866717
1415675_at 293.9302678
1415676_a_at 1478.074174
1415677_at 249.8853515
1415678_at 873.4318599
1415679_at 2409.001179
1415680_at 291.2964018
1415681_at 246.0742949
1415682_at 208.9525285
1415683_at 1021.545723
1415684_at 259.4457235
1415685_at 136.8298178
1415686_at 955.70363
1415687_a_at 1984.374538
1415688_at 1847.326976
1415689_s_at 468.4885806

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM180493.CEL.gz 2.9 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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