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Sample GSM180495 Query DataSets for GSM180495
Status Public on Dec 04, 2007
Title ActCD8TGF, rep2
Sample type RNA
 
Source name CD8 T cells activated in vitro with TGFbeta
Organism Mus musculus
Characteristics Genetic background: BDC2.5/NOD
Age: 4-6 wks
Number of mice per chip: 3-6
Tissue: Spleen and LN (cervical, mesenteric)
Sort: B220-, CD11b-, CD4-, CD69-, CD25-, CD62Lhi, CD8a+
Culture: Activation in vitro with anti-CD3/-CD28 beads + IL2 (20 U/ml) + TGFbeta (25 ng/ml) for 4 days
Post-culture sort: CD8a hi expressers
Extracted molecule total RNA
Extraction protocol RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
Label Biotin
Label protocol BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
 
Hybridization protocol Per Joslin Diabetes Center microarray core facility.
Scan protocol Per Joslin Diabetes Center microarray core facility.
Description jos3470.CEL
Data processing RMA
 
Submission date Apr 04, 2007
Last update date Aug 28, 2018
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL1261
Series (1)
GSE7460 Contribution of Foxp3 to the Treg signature
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE RMA-unlogged

Data table
ID_REF VALUE
1415670_at 150.9952183
1415671_at 572.8678612
1415672_at 3863.04347
1415673_at 252.2171521
1415674_a_at 299.5011779
1415675_at 281.8036216
1415676_a_at 1452.088581
1415677_at 237.9303078
1415678_at 891.4320283
1415679_at 2133.578262
1415680_at 209.2964629
1415681_at 257.223635
1415682_at 194.888623
1415683_at 801.8773539
1415684_at 245.0554134
1415685_at 90.35368703
1415686_at 791.1005255
1415687_a_at 2611.66507
1415688_at 1776.185933
1415689_s_at 561.5486822

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM180495.CEL.gz 2.9 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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