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Sample GSM180496 Query DataSets for GSM180496
Status Public on Dec 04, 2007
Title ActCD8TGF, rep3
Sample type RNA
 
Source name CD8 T cells activated in vitro with TGFbeta
Organism Mus musculus
Characteristics Genetic background: BDC2.5/NOD
Age: 4-6 wks
Number of mice per chip: 3-6
Tissue: Spleen and LN (cervical, mesenteric)
Sort: B220-, CD11b-, CD4-, CD69-, CD25-, CD62Lhi, CD8a+
Culture: Activation in vitro with anti-CD3/-CD28 beads + IL2 (20 U/ml) + TGFbeta (25 ng/ml) for 4 days
Post-culture sort: CD8a hi expressers
Extracted molecule total RNA
Extraction protocol RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
Label Biotin
Label protocol BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
 
Hybridization protocol Per Joslin Diabetes Center microarray core facility.
Scan protocol Per Joslin Diabetes Center microarray core facility.
Description jos3472.CEL
Data processing RMA
 
Submission date Apr 04, 2007
Last update date Aug 28, 2018
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL1261
Series (1)
GSE7460 Contribution of Foxp3 to the Treg signature
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE RMA-unlogged

Data table
ID_REF VALUE
1415670_at 151.6112668
1415671_at 626.4529782
1415672_at 4501.017152
1415673_at 303.3841812
1415674_a_at 359.971272
1415675_at 308.2885806
1415676_a_at 1485.538832
1415677_at 294.7305785
1415678_at 941.4742969
1415679_at 2174.032931
1415680_at 252.9885806
1415681_at 300.2265106
1415682_at 207.2824624
1415683_at 1116.821303
1415684_at 293.4827282
1415685_at 124.1610738
1415686_at 1153.96911
1415687_a_at 2621.813821
1415688_at 1893.704084
1415689_s_at 545.7457235

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM180496.CEL.gz 2.9 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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