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Sample GSM180497 Query DataSets for GSM180497
Status Public on Dec 04, 2007
Title FOXP3rv, rep2
Sample type RNA
 
Source name Tconv cells retrovirally transduced with FOXP3
Organism Mus musculus
Characteristics Genetic background: BDC2.5/NOD
Age: 4-6 wks
Number of mice per chip: 3-6
Tissue: Spleen and LN (cervical, mesenteric)
Sort: B220-, CD11b-, CD8a-, CD69-, CD62Lhi, CD4+, CD25-
Culture: Activation in vitro with anti-CD3/-CD28 beads + IL2 (20 U/ml) for 48hrs, then spin infected with retrovirus (FOXP3-thy1.1) and cultured for an additional 48hrs.
Post-culture sort: Thy1.1+ cells
Extracted molecule total RNA
Extraction protocol RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
Label Biotin
Label protocol BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
 
Hybridization protocol Per Joslin Diabetes Center microarray core facility.
Scan protocol Per Joslin Diabetes Center microarray core facility.
Description jos3286.CEL
Data processing RMA
 
Submission date Apr 04, 2007
Last update date Aug 28, 2018
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL1261
Series (1)
GSE7460 Contribution of Foxp3 to the Treg signature
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE RMA-unlogged

Data table
ID_REF VALUE
1415670_at 173.6802907
1415671_at 413.5981471
1415672_at 4765.970075
1415673_at 254.0159604
1415674_a_at 314.4253448
1415675_at 270.6807652
1415676_a_at 1163.017152
1415677_at 281.0921206
1415678_at 891.2381346
1415679_at 2159.51257
1415680_at 306.0117881
1415681_at 254.4907157
1415682_at 219.9600092
1415683_at 1021.603771
1415684_at 348.7798376
1415685_at 117.4990328
1415686_at 1415.162674
1415687_a_at 1575.998162
1415688_at 2149.733981
1415689_s_at 563.7395987

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM180497.CEL.gz 3.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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