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Sample GSM180498 Query DataSets for GSM180498
Status Public on Dec 04, 2007
Title FOXP3rv, rep3
Sample type RNA
 
Source name Tconv cells retrovirally transduced with FOXP3
Organism Mus musculus
Characteristics Genetic background: BDC2.5/NOD
Age: 4-6 wks
Number of mice per chip: 3-6
Tissue: Spleen and LN (cervical, mesenteric)
Sort: B220-, CD11b-, CD8a-, CD69-, CD62Lhi, CD4+, CD25-
Culture: Activation in vitro with anti-CD3/-CD28 beads + IL2 (20 U/ml) for 48hrs, then spin infected with retrovirus (FOXP3-thy1.1) and cultured for an additional 48hrs.
Post-culture sort: Thy1.1+ cells
Extracted molecule total RNA
Extraction protocol RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
Label Biotin
Label protocol BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
 
Hybridization protocol Per Joslin Diabetes Center microarray core facility.
Scan protocol Per Joslin Diabetes Center microarray core facility.
Description jos3323.CEL
Data processing RMA
 
Submission date Apr 04, 2007
Last update date Aug 28, 2018
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL1261
Series (1)
GSE7460 Contribution of Foxp3 to the Treg signature
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE RMA-unlogged

Data table
ID_REF VALUE
1415670_at 162.4578007
1415671_at 448.0348487
1415672_at 4379.596702
1415673_at 305.9970394
1415674_a_at 370.2278092
1415675_at 253.5201495
1415676_a_at 1563.158087
1415677_at 296.1782317
1415678_at 1036.831283
1415679_at 2412.179638
1415680_at 319.8034758
1415681_at 278.5844652
1415682_at 250.5355035
1415683_at 1024.131438
1415684_at 277.2100689
1415685_at 103.5168414
1415686_at 1202.920949
1415687_a_at 1783.19022
1415688_at 2206.249441
1415689_s_at 439.84566

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM180498.CEL.gz 3.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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