NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM180500 Query DataSets for GSM180500
Status Public on Dec 04, 2007
Title CTRLrv, rep2
Sample type RNA
 
Source name Tconv cells retrovirally transduced with empty vector
Organism Mus musculus
Characteristics Genetic background: BDC2.5/NOD
Age: 4-6 wks
Number of mice per chip: 3-6
Tissue: Spleen and LN (cervical, mesenteric)
Sort: B220-, CD11b-, CD8a-, CD69-, CD62Lhi, CD4+, CD25-
Culture: Activation in vitro with anti-CD3/-CD28 beads + IL2 (20 U/ml) for 48hrs, then spin infected with retrovirus (empty-thy1.1) and cultured for an additional 48hrs.
Post-culture sort: Thy1.1+ cells
Extracted molecule total RNA
Extraction protocol RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
Label Biotin
Label protocol BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
 
Hybridization protocol Per Joslin Diabetes Center microarray core facility.
Scan protocol Per Joslin Diabetes Center microarray core facility.
Description jos3285.CEL
Data processing RMA
 
Submission date Apr 04, 2007
Last update date Aug 28, 2018
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL1261
Series (1)
GSE7460 Contribution of Foxp3 to the Treg signature
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE RMA-unlogged

Data table
ID_REF VALUE
1415670_at 217.8423788
1415671_at 446.1742949
1415672_at 4081.64064
1415673_at 319.6760032
1415674_a_at 288.1658043
1415675_at 253.9982719
1415676_a_at 1175.114305
1415677_at 305.8728729
1415678_at 998.7679443
1415679_at 1925.288581
1415680_at 415.4493289
1415681_at 233.2028664
1415682_at 197.2739571
1415683_at 1147.395818
1415684_at 363.7242416
1415685_at 135.3307152
1415686_at 1225.384246
1415687_a_at 1657.250974
1415688_at 1855.460584
1415689_s_at 549.4104607

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM180500.CEL.gz 3.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap