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Sample GSM180506 Query DataSets for GSM180506
Status Public on Dec 04, 2007
Title Foxp3rv, rep2
Sample type RNA
 
Source name Tconv cells retroviarally transduced with Foxp3
Organism Mus musculus
Characteristics Genetic background: BDC2.5/NOD
Age: 4-6 wks
Number of mice per chip: 3-6
Tissue: Spleen and LN (cervical, mesenteric)
Sort: B220-, CD11b-, CD8a-, CD69-, CD62Lhi, CD4+, CD25-
Culture: Activation in vitro with anti-CD3/-CD28 beads + IL2 (20 U/ml) for 48hrs, then spin infected with retrovirus (Foxp3-GFP) and cultured for an additional 48hrs.
Post-culture sort: GFP+ cells
Extracted molecule total RNA
Extraction protocol RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
Label Biotin
Label protocol BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
 
Hybridization protocol Per Joslin Diabetes Center microarray core facility.
Scan protocol Per Joslin Diabetes Center microarray core facility.
Description jos3318.CEL
Data processing RMA
 
Submission date Apr 04, 2007
Last update date Aug 28, 2018
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL1261
Series (1)
GSE7460 Contribution of Foxp3 to the Treg signature
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE RMA-unlogged

Data table
ID_REF VALUE
1415670_at 152.652703
1415671_at 481.0742949
1415672_at 3823.254096
1415673_at 264.7359941
1415674_a_at 365.3291926
1415675_at 269.4646258
1415676_a_at 1391.275122
1415677_at 291.0996219
1415678_at 957.0437269
1415679_at 3157.512119
1415680_at 247.1985861
1415681_at 269.0457235
1415682_at 224.1822851
1415683_at 1011.701077
1415684_at 277.9604877
1415685_at 130.1644301
1415686_at 1267.79271
1415687_a_at 1716.369854
1415688_at 2249.575718
1415689_s_at 455.4031747

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM180506.CEL.gz 3.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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