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Sample GSM180508 Query DataSets for GSM180508
Status Public on Dec 04, 2007
Title Ctrlrv, rep2
Sample type RNA
Source name Tconv cells retrovirally transduced with empty vector
Organism Mus musculus
Characteristics Genetic background: BDC2.5/NOD
Age: 4-6 wks
Number of mice per chip: 3-6
Tissue: Spleen and LN (cervical, mesenteric)
Sort: B220-, CD11b-, CD8a-, CD69-, CD62Lhi, CD4+, CD25-
Culture: Activation in vitro with anti-CD3/-CD28 beads + IL2 (20 U/ml) for 48hrs, then spin infected with retrovirus (empty-GFP) and cultured for an additional 48hrs.
Post-culture sort: GFP+ cells
Extracted molecule total RNA
Extraction protocol RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
Label Biotin
Label protocol BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
Hybridization protocol Per Joslin Diabetes Center microarray core facility.
Scan protocol Per Joslin Diabetes Center microarray core facility.
Description jos3319.CEL
Data processing RMA
Submission date Apr 04, 2007
Last update date Aug 28, 2018
Contact name CBDM Lab
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
Platform ID GPL1261
Series (1)
GSE7460 Contribution of Foxp3 to the Treg signature
Reanalyzed by GSE119085

Data table header descriptions
VALUE RMA-unlogged

Data table
1415670_at 130.0084126
1415671_at 446.3417429
1415672_at 4624.985111
1415673_at 328.5329985
1415674_a_at 304.0414183
1415675_at 245.8051547
1415676_a_at 1409.35547
1415677_at 360.3240845
1415678_at 890.6392606
1415679_at 3205.611762
1415680_at 284.5962687
1415681_at 310.9196518
1415682_at 252.0393951
1415683_at 1089.302866
1415684_at 280.1482686
1415685_at 102.0719117
1415686_at 1129.892211
1415687_a_at 1639.604925
1415688_at 2403.783086
1415689_s_at 457.5218077

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.

Supplementary file Size Download File type/resource
GSM180508.CEL.gz 3.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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