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Sample GSM180510 Query DataSets for GSM180510
Status Public on Feb 15, 2008
Title AktTGF, rep2
Sample type RNA
 
Source name Tconv cells retrovirally transduced with constituatively active Akt and activated in vitro with TGFbeta
Organism Mus musculus
Characteristics Genetic background: BDC2.5/NOD
Age: 4-6 wks
Number of mice per chip: 3-6
Tissue: Spleen and LN (cervical, mesenteric)
Sort: B220-, CD11b-, CD8a-, CD69-, CD62Lhi, CD4+, CD25-
Culture: Activation in vitro with anti-CD3/-CD28 beads + IL2 (20 U/ml) + TGFbeta (25ng/ml) for 24hrs, then spin infected with retrovirus (Akt-GFP) and cultured for an additional 72hrs.
Post-culture sort: GFP+ cells
Extracted molecule total RNA
Extraction protocol RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
Label Biotin
Label protocol BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
 
Hybridization protocol Per Joslin Diabetes Center microarray core facility.
Scan protocol Per Joslin Diabetes Center microarray core facility.
Description jos3458.CEL
Data processing RMA
 
Submission date Apr 04, 2007
Last update date Aug 28, 2018
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL1261
Series (1)
GSE7596 AKT regulates de novo induction of Foxp3
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE RMA-unlogged

Data table
ID_REF VALUE
1415670_at 168.9524402
1415671_at 652.3222429
1415672_at 4201.272897
1415673_at 412.2884634
1415674_a_at 606.0060932
1415675_at 358.9241873
1415676_a_at 2127.713644
1415677_at 379.7665661
1415678_at 901.1499456
1415679_at 3786.710853
1415680_at 294.0608285
1415681_at 261.8772424
1415682_at 162.8600092
1415683_at 1292.006273
1415684_at 271.7458779
1415685_at 144.8773287
1415686_at 1199.827832
1415687_a_at 2232.191248
1415688_at 1882.162675
1415689_s_at 469.3828085

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM180510.CEL.gz 4.0 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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