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Sample GSM180513 Query DataSets for GSM180513
Status Public on Feb 15, 2008
Title AktTGF, rep3
Sample type RNA
 
Source name Tconv cells retrovirally transduced with constituatively active Akt and activated in vitro with TGFbeta
Organism Mus musculus
Characteristics Genetic background: BDC2.5/NOD
Age: 4-6 wks
Number of mice per chip: 3-6
Tissue: Spleen and LN (cervical, mesenteric)
Sort: B220-, CD11b-, CD8a-, CD69-, CD62Lhi, CD4+, CD25-
Culture: Activation in vitro with anti-CD3/-CD28 beads + IL2 (20 U/ml) + TGFbeta (25ng/ml) for 24hrs, then spin infected with retrovirus (Akt-GFP) and cultured for an additional 72hrs.
Post-culture sort: GFP+ cells
Extracted molecule total RNA
Extraction protocol RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
Label Biotin
Label protocol BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
 
Hybridization protocol Per Joslin Diabetes Center microarray core facility.
Scan protocol Per Joslin Diabetes Center microarray core facility.
Description jos3453.CEL
Data processing RMA
 
Submission date Apr 04, 2007
Last update date Aug 28, 2018
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL1261
Series (1)
GSE7596 AKT regulates de novo induction of Foxp3
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE RMA-unlogged

Data table
ID_REF VALUE
1415670_at 150.4199892
1415671_at 640.7615985
1415672_at 4519.923667
1415673_at 425.7716129
1415674_a_at 542.1054621
1415675_at 363.8395839
1415676_a_at 2020.931438
1415677_at 494.286572
1415678_at 823.6084283
1415679_at 3788.508018
1415680_at 267.945757
1415681_at 275.3086476
1415682_at 179.3214194
1415683_at 1331.690548
1415684_at 313.5718521
1415685_at 135.5600124
1415686_at 1143.827701
1415687_a_at 1996.582217
1415688_at 1980.668624
1415689_s_at 479.8109598

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM180513.CEL.gz 4.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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