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Sample GSM180515 Query DataSets for GSM180515
Status Public on Feb 15, 2008
Title GFPTGF, rep2
Sample type RNA
 
Source name Tconv cells retrovirally transduced with empty vector and activated in vitro with TGFbeta
Organism Mus musculus
Characteristics Genetic background: BDC2.5/NOD
Age: 4-6 wks
Number of mice per chip: 3-6
Tissue: Spleen and LN (cervical, mesenteric)
Sort: B220-, CD11b-, CD8a-, CD69-, CD62Lhi, CD4+, CD25-
Culture: Activation in vitro with anti-CD3/-CD28 beads + IL2 (20 U/ml) + TGFbeta (25ng/ml) for 24hrs, then spin infected with retrovirus (empty-GFP) and cultured for an additional 72hrs.
Post-culture sort: GFP+ cells
Extracted molecule total RNA
Extraction protocol RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
Label Biotin
Label protocol BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
 
Hybridization protocol Per Joslin Diabetes Center microarray core facility.
Scan protocol Per Joslin Diabetes Center microarray core facility.
Description jos3452.CEL
Data processing RMA
 
Submission date Apr 04, 2007
Last update date Aug 28, 2018
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL1261
Series (1)
GSE7596 AKT regulates de novo induction of Foxp3
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE RMA-unlogged

Data table
ID_REF VALUE
1415670_at 169.0628841
1415671_at 533.4937076
1415672_at 4254.374295
1415673_at 332.2885806
1415674_a_at 490.0929236
1415675_at 345.1813405
1415676_a_at 2209.202866
1415677_at 270.2483084
1415678_at 1017.915398
1415679_at 2997.166896
1415680_at 310.7598016
1415681_at 281.0812058
1415682_at 216.5296988
1415683_at 1258.002866
1415684_at 358.3683724
1415685_at 145.1850617
1415686_at 1173.091738
1415687_a_at 1494.527167
1415688_at 2068.132066
1415689_s_at 442.2233425

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM180515.CEL.gz 4.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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