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Sample GSM180516 Query DataSets for GSM180516
Status Public on Feb 15, 2008
Title GFPTGF, rep3
Sample type RNA
 
Source name Tconv cells retrovirally transduced with empty vector and activated in vitro with TGFbeta
Organism Mus musculus
Characteristics Genetic background: BDC2.5/NOD
Age: 4-6 wks
Number of mice per chip: 3-6
Tissue: Spleen and LN (cervical, mesenteric)
Sort: B220-, CD11b-, CD8a-, CD69-, CD62Lhi, CD4+, CD25-
Culture: Activation in vitro with anti-CD3/-CD28 beads + IL2 (20 U/ml) + TGFbeta (25ng/ml) for 24hrs, then spin infected with retrovirus (empty-GFP) and cultured for an additional 72hrs.
Post-culture sort: GFP+ cells
Extracted molecule total RNA
Extraction protocol RNA isolated with Trizol reagent and amplified twice with the MessageAmp aRNA Kit (Ambion)
Label Biotin
Label protocol BioArray High Yield RNA Transcript Labeling T7 Kit (Enzo Life Sciences)
 
Hybridization protocol Per Joslin Diabetes Center microarray core facility.
Scan protocol Per Joslin Diabetes Center microarray core facility.
Description jos3457.CEL
Data processing RMA
 
Submission date Apr 04, 2007
Last update date Aug 28, 2018
Contact name CBDM Lab
E-mail(s) cbdm@hms.harvard.edu
Phone 617-432-7747
Organization name Harvard Medical School
Department Microbiology and Immunobiology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platform ID GPL1261
Series (1)
GSE7596 AKT regulates de novo induction of Foxp3
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE RMA-unlogged

Data table
ID_REF VALUE
1415670_at 170.8600092
1415671_at 566.0352513
1415672_at 4445.935782
1415673_at 352.3085282
1415674_a_at 522.1501621
1415675_at 334.4722053
1415676_a_at 2411.808295
1415677_at 310.9750862
1415678_at 1002.646872
1415679_at 3058.334773
1415680_at 307.8283363
1415681_at 269.7918357
1415682_at 239.6270671
1415683_at 1341.040172
1415684_at 316.254666
1415685_at 157.4240954
1415686_at 1278.587139
1415687_a_at 1594.341828
1415688_at 2030.882129
1415689_s_at 410.6257815

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM180516.CEL.gz 4.0 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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