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Sample GSM181345 Query DataSets for GSM181345
Status Public on Mar 29, 2008
Title Tcell_18h_TGF_rep2
Sample type RNA
 
Source name T cells resting, 18h + 10ng TGF
Organism Homo sapiens
Characteristics T cells resting, 18h + 10ng TGF
Extracted molecule total RNA
Extraction protocol CD4+ T cells were isolated from blood samples by using RosetteSep CD4+ enrichment kit (StemCell Technologies, Meylan, France); purity: > 90% as determined by flow cytometry. RNA was isolated with Trizol(r) method according to the manufacturer's protocol. The RNA was purified with the RNeasy MiniElute Cleanup Kit (Qiagen, Hilden, Germany).
Label biotin
Label protocol Biotin labeled cRNA preparation for the Illumina platform was performed using the Ambion(r) Illumina RNA amplification kit (Ambion Europe, Huntington, Cambridgeshire, UK).
 
Hybridization protocol Biotin labeled cRNA (1.5ug) was hybridized to Sentrix(r) whole genome bead chips 6 x 2 (Illumina, San Diego, CA, USA)
Scan protocol Scanning was performed on the Illumina(r) BeadStation 500x.
Description Tcell_18h_TGF_rep2
Data processing BeadStudio raw data were normalized using R
 
Submission date Apr 09, 2007
Last update date Dec 19, 2012
Contact name Joachim Schultze
E-mail(s) j.schultze@uni-bonn.de
Organization name LIMES (Life and Medical Sciences Center Genomics and Immunoregulation)
Department Genomics and Immunoregulation
Street address Carl-Troll-Strasse 31
City Bonn
State/province NRW
ZIP/Postal code 53115
Country Germany
 
Platform ID GPL2507
Series (1)
GSE7497 Influence of TGFbeta on human resting CD4+ T cells

Data table header descriptions
ID_REF
VALUE quantiles normalized value 18h+10ngTGF

Data table
ID_REF VALUE
GI_10047089-S 56.42222222
GI_10047091-S 121.4925926
GI_10047093-S 227.3074074
GI_10047099-S 82.71481481
GI_10047103-S 2005.62963
GI_10047105-S 89.38518519
GI_10047121-S 66.33333333
GI_10047123-S 212.262963
GI_10047133-A 84.53333333
GI_10047133-I 81.75555556
GI_10092578-S 68.81481481
GI_10092585-S 188.9888889
GI_10092596-S 166.8407407
GI_10092600-S 148.7555556
GI_10092602-S 90.02222222
GI_10092603-S 82.50740741
GI_10092611-A 206.8851852
GI_10092616-S 81.15925926
GI_10092618-S 1096.87037
GI_10092638-S 79.86296296

Total number of rows: 47293

Table truncated, full table size 1130 Kbytes.




Supplementary file Size Download File type/resource
GSM181345_raw.txt.gz 310.7 Kb (ftp)(http) TXT
Processed data included within Sample table

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