|
| Status |
Public on May 01, 2007 |
| Title |
Tcell_18h_1h_TGF_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
T cells resting, 18h + 1h + 10ng TGF
|
| Organism |
Homo sapiens |
| Characteristics |
T cells resting, 18h + 1h + 10ng TGF
|
| Extracted molecule |
total RNA |
| Extraction protocol |
CD4+ T cells were isolated from blood samples by using RosetteSep CD4+ enrichment kit (StemCell Technologies, Meylan, France); purity: > 90% as determined by flow cytometry. RNA was isolated with Trizol(r) method according to the manufacturer's protocol. The RNA was purified with the RNeasy MiniElute Cleanup Kit (Qiagen, Hilden, Germany).
|
| Label |
biotin
|
| Label protocol |
Biotin labeled cRNA preparation for the Illumina platform was performed using the Ambion(r) Illumina RNA amplification kit (Ambion Europe, Huntington, Cambridgeshire, UK).
|
| |
|
| Hybridization protocol |
Biotin labeled cRNA (1.5ug) was hybridized to Sentrix(r) whole genome bead chips 6 x 2 (Illumina, San Diego, CA, USA)
|
| Scan protocol |
Scanning was performed on the Illumina(r) BeadStation 500x.
|
| Description |
Tcell_18h_1h_TGF_rep1
|
| Data processing |
BeadStudio raw data were normalized using R
|
| |
|
| Submission date |
Apr 09, 2007 |
| Last update date |
Dec 19, 2012 |
| Contact name |
Joachim Schultze |
| E-mail(s) |
j.schultze@uni-bonn.de
|
| Organization name |
LIMES (Life and Medical Sciences Center Genomics and Immunoregulation)
|
| Department |
Genomics and Immunoregulation
|
| Street address |
Carl-Troll-Strasse 31
|
| City |
Bonn |
| State/province |
NRW |
| ZIP/Postal code |
53115 |
| Country |
Germany |
| |
|
| Platform ID |
GPL2507 |
| Series (1) |
| GSE7497 |
Influence of TGFbeta on human resting CD4+ T cells |
|
