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Sample GSM1820503 Query DataSets for GSM1820503
Status Public on Jul 12, 2016
Title DRG_ob/+_ 5
Sample type RNA
Source name dorsal root ganglia, female, 26 week, control
Organism Mus musculus
Characteristics gender: female
genotype/variation: ob/+
tissue: dorsal root ganglia
Treatment protocol N/A
Growth protocol N/A
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from dorsal root ganglia and sciatic nerve biopsies using a commercially available kit (RNeasy Mini Kit; QIAGEN, Inc., Valencia, CA), including an on-column deoxyribonuclease digestion and following the manufacturer’s protocol. RNA quality and quantity were assessed by microfluid electrophoresis using an RNA 6000 Pico LabChip on a 2100 Bioanalyzer (Agilent Technologies, Inc., Santa Clara, CA).
Label biotin
Label protocol Biotinylated cDNA were prepared according to the Nugen Ovation V2 kit protocol from 75 ng total RNA (Ovation RNA Amplification System V2 Manual, 2007, NuGEN).
Hybridization protocol Following Labeling, 4 ug of cDNA were hybridized for 16 hr at 45C on GeneChip Mouse 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader.
Description gene expression data
Data processing The Affymetrix CEL files were initially analyzed using a local version of the GenePattern genomic analysis platform from the Broad Institute. The samples were Robust Multi-array Average (RMA) normalized using the BrainArray Custom CDF 16 Entrez.
Submission date Jul 13, 2015
Last update date Jul 12, 2016
Contact name Junguk Hur
Organization name University of North Dakota
Department Biomedical Sciences
Street address 1301 N. Columbia Rd. Stop 9037
City Grand Forks
State/province ND
ZIP/Postal code 58202-9037
Country USA
Platform ID GPL16368
Series (1)
GSE70852 Dorsal root ganglia (DRG) and Sciatic nerve (SCN) in female BTBR ob/ob mice - diabetic peripheral neuropathy

Data table header descriptions
VALUE RMA signal - BrainArray Custom CDF v16 Entrez.

Data table
100008567_at 663.13
100009600_at 112.88
100012_at 9.31
100017_at 72.97
100019_at 583.31
100034251_at 135.10
100034739_at 15.50
100034748_at 45.03
100036520_at 57.91
100036521_at 389.26
100036523_at 23.71
100036768_at 42.41
100037258_at 1156.12
100037262_at 26.26
100037278_at 47.98
100038347_at 772.39
100038358_at 19.02
100038371_at 115.70
100038392_at 34.26
100038398_at 31.34

Total number of rows: 17565

Table truncated, full table size 280 Kbytes.

Supplementary file Size Download File type/resource
GSM1820503_EF2011-65_DRG-cont.CEL.gz 6.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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