|
| Status |
Public on Jan 02, 2008 |
| Title |
KO_RA+LIF_8hr_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
CYP26A1-/- ES cells, 100 nM RA +LIF, 8hr
|
| Organism |
Mus musculus |
| Characteristics |
CYP26A1-/- AB1 murine ES cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using RNA-STAT-60 (Tel-test, #Cs-112). The GeneChip Expression 3'-Amplification Reagents One-Cycle cDNA Synthesis Kit (P/N 900431) was used subsequently to generate cDNA from the total RNA. The cDNA was used for in vitro transcription to form complementary RNA (cRNA), which was then labeled with biotin.
|
| Label |
biotin
|
| Label protocol |
The IVT labeling kit (part of the One-Cycle cDNA Synthesis Kit) was used to biotin-label the cRNA.
|
| |
|
| Hybridization protocol |
The hybridization steps were performed by the Microarray Core Facility of Weill Medical College of Cornell University, using the Affymetrix platform (Hyb Oven 640)
|
| Scan protocol |
The scanning was performed by the Microarray Core Facility of Weill Medical College of Cornell University, using the Affymetrix platform (7G 3000 and Fluidics Station).
|
| Description |
After biotin-labeling, the GeneChip Sample Cleanup Module was used to prepare the biotinylated cRNA for hybridization. Following the standard protocol, the biotinylated cRNA was also fragmented prior to hybridization.
|
| Data processing |
MAS 5.0
|
| |
|
| Submission date |
Apr 16, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Simne Langton |
| Phone |
212-746-6262
|
| Organization name |
Weill Cornell Medical College
|
| Department |
Pharmacology
|
| Lab |
Lorraine J. Gudas
|
| Street address |
1300 York Ave, E405
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10021 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE7528 |
Gene expression of Wt vs CYP26A1-/- murine ES cells treated with control or 100 nM RA for 8 or 72 hr. |
|
| Relations |
| Reanalyzed by |
GSE119085 |
