|
| Status |
Public on Jun 20, 2007 |
| Title |
B10.BR naive rep1 |
| Sample type |
RNA |
| |
|
| Source name |
lymph node
|
| Organism |
Mus musculus |
| Characteristics |
B10.BR-CD4 naive
|
| Treatment protocol |
lymph node was extracted and single cell suspension made sorted by flow cytometry
|
| Growth protocol |
none
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen column as described by the manufacturer's protocol
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to Ambion protocol from 10 ug total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized on GeneChip mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
genechips were scanned using GSI Lumonics scanner
|
| Description |
Gene expression data from B10.BR naive mouse
|
| Data processing |
GC-RMA normalized data via Genespring GX software
|
| |
|
| Submission date |
Apr 18, 2007 |
| Last update date |
Mar 18, 2009 |
| Contact name |
Michael McHeyzer-Williams |
| Organization name |
The Scripps Research Institute
|
| Street address |
10550 North Torrey Pines Road
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL8321 |
| Series (1) |
| GSE7548 |
Expression data from immunized B10.BR mice |
|
