|
Status |
Public on Jun 20, 2007 |
Title |
B10.BR naive rep2 |
Sample type |
RNA |
|
|
Source name |
lymph node
|
Organism |
Mus musculus |
Characteristics |
B10.BR-CD4 naive
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiagen column as described by the manufacturer's protocol
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to Ambion protocol from 10 ug total RNA.
|
|
|
Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized on GeneChip mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
Scan protocol |
genechips were scanned using GSI Lumonics scanner
|
Description |
Gene expression data from B10.BR naive mouse
|
Data processing |
GC-RMA normalized data via Genespring GX software
|
|
|
Submission date |
Apr 18, 2007 |
Last update date |
Mar 18, 2009 |
Contact name |
Michael McHeyzer-Williams |
Organization name |
The Scripps Research Institute
|
Street address |
10550 North Torrey Pines Road
|
City |
La Jolla |
State/province |
CA |
ZIP/Postal code |
92037 |
Country |
USA |
|
|
Platform ID |
GPL8321 |
Series (1) |
GSE7548 |
Expression data from immunized B10.BR mice |
|