|
| Status |
Public on Dec 06, 2016 |
| Title |
Kidney marrow_Control-zebrafish_5 |
| Sample type |
RNA |
| |
|
| Source name |
Kidney marrow_Control-zebrafish
|
| Organism |
Danio rerio |
| Characteristics |
genotype/variation: control
tissue: kidney marrow
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (GeneChip® 3’ IVT Express Kit Technical Manual, 2008, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000
|
| Data processing |
Microarray data (.CEL files) were normalized using the justRMA algorithm and analyzed using R-Bioconductor (Version 2.15.3)
|
| |
|
| Submission date |
Jul 23, 2015 |
| Last update date |
Dec 07, 2016 |
| Contact name |
Chiara Borga |
| E-mail(s) |
chiaraborga@gmail.com
|
| Organization name |
University of Padua
|
| Street address |
Corso Stati Uniti,4
|
| City |
Padova |
| ZIP/Postal code |
35127 |
| Country |
Italy |
| |
|
| Platform ID |
GPL1319 |
| Series (1) |
| GSE71270 |
Creb overexpression induces leukemia in zebrafish by blocking myeloid differentiation process |
|
