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Sample GSM183755 Query DataSets for GSM183755
Status Public on Jan 01, 2008
Title 2088_z_r1
Sample type RNA
 
Source name E10.5 mouse limb bud
Organism Mus musculus
Characteristics GFP-positive, ZPA
Treatment protocol After dissociating the limbs into single cell suspensions, GFP-positive (ZPA) and GFP-negative (rest of the limb) cells were purified by FACS.
Growth protocol Shhgfpcre heterozygotes were harvested at E10.5.
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy according to manufacturer's instructions
Label biotin
Label protocol Biotinylated cRNA was prepared according to the two-cycle Affymetrix protocol from 50ng total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
 
Hybridization protocol Following fragmentation, 15 ug of cRNA was hybridized for 16 hr at 45C on Mu430 2.0 A Array. GeneChips were processed on GeneChip fluidics station F450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Description gene expression from ZPA cells
Data processing The data were analyzed with GCOS 1.3 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
 
Submission date Apr 24, 2007
Last update date Aug 28, 2018
Contact name Brian Harfe
E-mail(s) bharfe@mgm.ufl.edu
Organization name University of Florida
Department Molecular Genetics and Microbiology
Street address 1376 Mowry Road
City Gainesville
State/province FL
ZIP/Postal code 32610
Country USA
 
Platform ID GPL1261
Series (1)
GSE7598 Identification and characterization of genes expressed in the mouse ZPA using a novel microarray approach
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 5666.71 P 0.000126798
AFFX-BioB-M_at 8686.4 P 4.42873e-05
AFFX-BioB-3_at 5428.75 P 4.42873e-05
AFFX-BioC-5_at 12442.8 P 5.16732e-05
AFFX-BioC-3_at 11246.8 P 5.16732e-05
AFFX-BioDn-5_at 14215.6 P 4.42873e-05
AFFX-BioDn-3_at 52584.4 P 4.42873e-05
AFFX-CreX-5_at 125404 P 5.16732e-05
AFFX-CreX-3_at 165083 P 4.42873e-05
AFFX-DapX-5_at 30.9251 A 0.5
AFFX-DapX-M_at 70.8586 A 0.58862
AFFX-DapX-3_at 12.7905 A 0.945802
AFFX-LysX-5_at 114.538 A 0.185131
AFFX-LysX-M_at 15.1655 A 0.891041
AFFX-LysX-3_at 10.8971 A 0.672921
AFFX-PheX-5_at 80.3364 A 0.814869
AFFX-PheX-M_at 7.36668 A 0.921998
AFFX-PheX-3_at 19.8987 A 0.824672
AFFX-ThrX-5_at 63.6021 A 0.672935
AFFX-ThrX-M_at 5.65152 A 0.953547

Total number of rows: 45101

Table truncated, full table size 1357 Kbytes.




Supplementary file Size Download File type/resource
GSM183755.CEL.gz 5.3 Mb (ftp)(http) CEL
GSM183755.EXP.gz 491 b (ftp)(http) EXP
Raw data provided as supplementary file
Processed data included within Sample table

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