|
| Status |
Public on Jan 01, 2008 |
| Title |
2100_n_r4 |
| Sample type |
RNA |
| |
|
| Source name |
E10.5 mouse limb bud
|
| Organism |
Mus musculus |
| Characteristics |
GFP-negative, rest of limb
|
| Treatment protocol |
After dissociating the limbs into single cell suspensions, GFP-positive (ZPA) and GFP-negative (rest of the limb) cells were purified by FACS.
|
| Growth protocol |
Shhgfpcre heterozygotes were harvested at E10.5.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy according to manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to the two-cycle Affymetrix protocol from 50ng total RNA (Expression Analysis Technical Manual, 2003, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA was hybridized for 16 hr at 45C on Mu430 2.0 A Array. GeneChips were processed on GeneChip fluidics station F450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
gene expression from cells outside of the ZPA
|
| Data processing |
The data were analyzed with GCOS 1.3 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Apr 24, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Brian Harfe |
| E-mail(s) |
bharfe@mgm.ufl.edu
|
| Organization name |
University of Florida
|
| Department |
Molecular Genetics and Microbiology
|
| Street address |
1376 Mowry Road
|
| City |
Gainesville |
| State/province |
FL |
| ZIP/Postal code |
32610 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE7598 |
Identification and characterization of genes expressed in the mouse ZPA using a novel microarray approach |
|
| Relations |
| Reanalyzed by |
GSE119085 |
