|
| Status |
Public on Nov 20, 2007 |
| Title |
sensitive1f2 |
| Sample type |
RNA |
| |
|
| Source name |
whole body of 3-5 day old females, sensitive line 1 replicate 2
|
| Organism |
Drosophila melanogaster |
| Characteristics |
3-5 day old females, sensitive line 1 replicate 2
|
| Biomaterial provider |
laboratory of Trudy Mackay
|
| Treatment protocol |
flies have been exposed to ethanol vapor prior to RNA extraction
|
| Growth protocol |
under standard conditions 25C, 12:12 light/dark cycle
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions
|
| Label |
Biotinylated cRNA
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 mkg of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Overall design: we performed 35 generations of artificial selection from a genetically heterogeneous base population to derive replicate lines that are sensitive or resistant to ethanol exposure, as well as unselected control lines.
At generation 25, two replicates of 15 3-5 day old virgin males and females were collected from each selection line.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500. Scale factor is 2.443
|
| |
|
| Submission date |
Apr 24, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Tatiana V Morozova |
| E-mail(s) |
tvmorozo@ncsu.edu
|
| Organization name |
NCSU
|
| Department |
zoology
|
| Lab |
Anholt
|
| Street address |
campus box 7617
|
| City |
Raleigh |
| State/province |
NC |
| ZIP/Postal code |
27695 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE7614 |
Phenotypic and transcriptional response to selection for alcohol sensitivity in Drosophila melanogaster |
|
| Relations |
| Reanalyzed by |
GSE119084 |
