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Sample GSM183972 Query DataSets for GSM183972
Status Public on Nov 01, 2007
Title ControlHeart_LPS_3h_rep2
Sample type RNA
 
Source name Heart,LPS,3h
Organism Mus musculus
Characteristics Strain:C57BL/6J,Gender:Female,Age44days
Treatment protocol Hearts were homogenized in Trizol immediately following dissection.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol RNA was labeled using the MessageAmp II-Biotin Enhanced Single Round RNA Amplification Kit (Perkin-Elmer Inc.)
 
Hybridization protocol Samples were hybridized to the Affymetrix Mouse 430 2.0 array using standard Affymetrix protocols.
Scan protocol The chips were scanned using the Affymetrix ScanArray 3000 using default settings.
Description Expression data from heart
Data processing A target intensity of 250 was used for scaling. Chips were included with a background less than 100 intensity units and a GAPDH 3':5'<3. The raw expression values were normalised using Robust Multichip Average. All further processing of the data were performed within the Bioconductor project and the R program software.
 
Submission date Apr 24, 2007
Last update date Aug 28, 2018
Contact name Yu Xia
E-mail(s) yuxia@scripps.edu
Phone 858-784-7364
Organization name The Scripps Research Institute
Department Genetics
Lab Bruce Beutler
Street address 10550, N. Torrey Pines Rd.
City La Jolla
State/province CA
ZIP/Postal code 92037
Country USA
 
Platform ID GPL1261
Series (1)
GSE7605 Expression in Kir6.1-deficient heart following LPS challenge
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE RMA values.

Data table
ID_REF VALUE
1415670_at 7.6489
1415671_at 9.486873
1415672_at 9.882499
1415673_at 5.684867
1415674_a_at 8.554185
1415675_at 7.590556
1415676_a_at 10.669557
1415677_at 8.216103
1415678_at 10.454367
1415679_at 10.415629
1415680_at 7.304144
1415681_at 9.818435
1415682_at 6.493154
1415683_at 10.303626
1415684_at 7.203417
1415685_at 8.873327
1415686_at 9.054659
1415687_a_at 12.305348
1415688_at 10.488568
1415689_s_at 8.493638

Total number of rows: 45101

Table truncated, full table size 895 Kbytes.




Supplementary file Size Download File type/resource
GSM183972.CEL.gz 6.1 Mb (ftp)(http) CEL
GSM183972.EXP.gz 517 b (ftp)(http) EXP
Processed data included within Sample table

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