|
| Status |
Public on Nov 01, 2007 |
| Title |
MutantHeart_PBS_3h_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Heart,PBS,3h
|
| Organism |
Mus musculus |
| Characteristics |
Strain:C57BL/6J,Gender:Female,Age44days
|
| Treatment protocol |
Hearts were homogenized in Trizol immediately following dissection.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
RNA was labeled using the MessageAmp II-Biotin Enhanced Single Round RNA Amplification Kit (Perkin-Elmer Inc.)
|
| |
|
| Hybridization protocol |
Samples were hybridized to the Affymetrix Mouse 430 2.0 array using standard Affymetrix protocols.
|
| Scan protocol |
The chips were scanned using the Affymetrix ScanArray 3000 using default settings.
|
| Description |
Expression data from heart
|
| Data processing |
A target intensity of 250 was used for scaling. Chips were included with a background less than 100 intensity units and a GAPDH 3':5'<3. The raw expression values were normalised using Robust Multichip Average. All further processing of the data were performed within the Bioconductor project and the R program software.
|
| |
|
| Submission date |
Apr 24, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Yu Xia |
| E-mail(s) |
yuxia@scripps.edu
|
| Phone |
858-784-7364
|
| Organization name |
The Scripps Research Institute
|
| Department |
Genetics
|
| Lab |
Bruce Beutler
|
| Street address |
10550, N. Torrey Pines Rd.
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE7605 |
Expression in Kir6.1-deficient heart following LPS challenge |
|
| Relations |
| Reanalyzed by |
GSE119085 |
