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Sample GSM183983 Query DataSets for GSM183983
Status Public on Nov 01, 2007
Title ControlHeart_LPS_30min_rep1
Sample type RNA
 
Source name Heart,LPS,30min
Organism Mus musculus
Characteristics Strain:C57BL/6J,Gender:Female,Age44days
Treatment protocol Hearts were homogenized in Trizol immediately following dissection.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol RNA was labeled using the MessageAmp II-Biotin Enhanced Single Round RNA Amplification Kit (Perkin-Elmer Inc.)
 
Hybridization protocol Samples were hybridized to the Affymetrix Mouse 430 2.0 array using standard Affymetrix protocols.
Scan protocol The chips were scanned using the Affymetrix ScanArray 3000 using default settings.
Description Expression data from heart
Data processing A target intensity of 250 was used for scaling. Chips were included with a background less than 100 intensity units and a GAPDH 3':5'<3. The raw expression values were normalised using Robust Multichip Average. All further processing of the data were performed within the Bioconductor project and the R program software.
 
Submission date Apr 24, 2007
Last update date Aug 28, 2018
Contact name Yu Xia
E-mail(s) yuxia@scripps.edu
Phone 858-784-7364
Organization name The Scripps Research Institute
Department Genetics
Lab Bruce Beutler
Street address 10550, N. Torrey Pines Rd.
City La Jolla
State/province CA
ZIP/Postal code 92037
Country USA
 
Platform ID GPL1261
Series (1)
GSE7605 Expression in Kir6.1-deficient heart following LPS challenge
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE RMA values.

Data table
ID_REF VALUE
1415670_at 7.87601
1415671_at 9.592362
1415672_at 9.985606
1415673_at 6.184062
1415674_a_at 8.635723
1415675_at 7.630352
1415676_a_at 10.770419
1415677_at 8.723062
1415678_at 10.428002
1415679_at 10.616062
1415680_at 6.875315
1415681_at 9.845601
1415682_at 7.080685
1415683_at 10.319507
1415684_at 7.609482
1415685_at 9.147141
1415686_at 9.116393
1415687_a_at 12.152096
1415688_at 10.618997
1415689_s_at 8.901533

Total number of rows: 45101

Table truncated, full table size 895 Kbytes.




Supplementary file Size Download File type/resource
GSM183983.CEL.gz 5.8 Mb (ftp)(http) CEL
GSM183983.EXP.gz 525 b (ftp)(http) EXP
Processed data included within Sample table

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