|
Status |
Public on Nov 01, 2007 |
Title |
ControlHeart_LPS_30min_rep1 |
Sample type |
RNA |
|
|
Source name |
Heart,LPS,30min
|
Organism |
Mus musculus |
Characteristics |
Strain:C57BL/6J,Gender:Female,Age44days
|
Treatment protocol |
Hearts were homogenized in Trizol immediately following dissection.
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
RNA was labeled using the MessageAmp II-Biotin Enhanced Single Round RNA Amplification Kit (Perkin-Elmer Inc.)
|
|
|
Hybridization protocol |
Samples were hybridized to the Affymetrix Mouse 430 2.0 array using standard Affymetrix protocols.
|
Scan protocol |
The chips were scanned using the Affymetrix ScanArray 3000 using default settings.
|
Description |
Expression data from heart
|
Data processing |
A target intensity of 250 was used for scaling. Chips were included with a background less than 100 intensity units and a GAPDH 3':5'<3. The raw expression values were normalised using Robust Multichip Average. All further processing of the data were performed within the Bioconductor project and the R program software.
|
|
|
Submission date |
Apr 24, 2007 |
Last update date |
Aug 28, 2018 |
Contact name |
Yu Xia |
E-mail(s) |
yuxia@scripps.edu
|
Phone |
858-784-7364
|
Organization name |
The Scripps Research Institute
|
Department |
Genetics
|
Lab |
Bruce Beutler
|
Street address |
10550, N. Torrey Pines Rd.
|
City |
La Jolla |
State/province |
CA |
ZIP/Postal code |
92037 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE7605 |
Expression in Kir6.1-deficient heart following LPS challenge |
|
Relations |
Reanalyzed by |
GSE119085 |