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Sample GSM184134 Query DataSets for GSM184134
Status Public on Jun 05, 2007
Title Lymphoma_TKO_8
Sample type RNA
 
Channel 1
Source name Atm-/-,mTerc-/-,p53-/- mouse lymphoma
Organism Mus musculus
Characteristics Strain:C57BL/6; lymphoma
Extracted molecule total RNA
Extraction protocol RNA was prepared by an initial extraction with Trizol (Invitrogen) according to the manufacturer’s instructions. Pelleted total RNA was then digested with RQ1 DNase (Promega) and subsequently purified through RNA purification columns (Gentra).
Label cy3, cy5
Label protocol Labeling to Agilent CGH arrays were performed as per manufacturer’s protocol (http://www.agilent.com)
 
Channel 2
Source name normal mouse reference
Organism Mus musculus
Characteristics Strain:C57BL/6
Extracted molecule total RNA
Extraction protocol RNA was prepared by an initial extraction with Trizol (Invitrogen) according to the manufacturer’s instructions. Pelleted total RNA was then digested with RQ1 DNase (Promega) and subsequently purified through RNA purification columns (Gentra).
Label cy5, cy3
Label protocol Labeling to Agilent CGH arrays were performed as per manufacturer’s protocol (http://www.agilent.com)
 
 
Hybridization protocol RNA hybridization to Agilent CGH arrays were performed as per manufacturer’s protocol (http://www.agilent.com)
Scan protocol Scanning was performed as per manufacturer's protocol (http://www.agilent.com)
Description lymphoma sample from Atm-/-, mTerc-/-, p53-/- triple knock-out mice were compared to 3 week old thymus RNA from healthy mice of p53 Hets
Data processing LOWESS normalized, background subtracted data obtained from log2 (test/reference). Agilent software was used.
 
Submission date Apr 24, 2007
Last update date Sep 14, 2007
Contact name bin feng
Organization name dfci
Street address 44 binney street
City boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL2872
Series (2)
GSE7607 Atm-/-,mTerc-/-,p53-/- triple knock-out lymphoma vs normal mouse DNA (GPL2872)
GSE7615 Cancer Process Study

Data table header descriptions
ID_REF
VALUE normalized log2 ratio (test/reference)

Data table
ID_REF VALUE
15097
37277 0.038232768
27972
17369 -0.102433407
26565 0.068320292
19729 0.025165054
29645 -0.056843996
39661 0.073245007
35728 0.003387463
22523
2044 0.426208236
29750 0.080757552
17500 -0.077121137
5706 -0.252291206
26573 -0.272507092
6471 0.042476843
13537 0.306799864
29681 0.017587433
33016 0.175471463
24539 -0.717862265

Total number of rows: 41175

Table truncated, full table size 695 Kbytes.




Supplementary file Size Download File type/resource
GSM184134_US23502418_251269416232_S01_44k.txt.gz 11.5 Mb (ftp)(http) TXT
GSM184134_US23502418_251269416285_S01_44k.txt.gz 11.5 Mb (ftp)(http) TXT
Processed data included within Sample table

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