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Sample GSM1842177 Query DataSets for GSM1842177
Status Public on Aug 04, 2015
Title Normal group C
Sample type RNA
 
Source name normal group
Organism Homo sapiens
Characteristics subject group: patients with cataract undergoing cataract surgery
mean age: 68.0 years
gender (male: female) ratio in the group: 3:2
tissue: Aqueous humor
Extracted molecule total RNA
Extraction protocol At the beginning of the surgery the aqueous humor was collected as previously reported. After a corneal paracentesis, a 30-gauge needle on a tuberculin microsyringe was used to aspirate aqueous humor from the anterior chamber, not contacting any iris or lens tissue during the whole sample collection. Approximately 0.1-0.2ml of aqueous humor was collected from each patient, transferred to 1.5ml tubes, and immediately placed on dry ice. Then the samples were subsequently stored at −80°C until analysis.
Label Hy3
Label protocol Total RNAs (100 ng) were hy3-labeled using the Low Input Quick Amp Labeling Kit, one-color (Agilent) according to the manufacturer's instructions.
 
Hybridization protocol The samples were labeled using the miRCURY™ Hy3™/Hy5™ Power labeling kit (Exiqon, Vedbaek, Denmark) and hybridized on the specific miRCURY ™ LNA Array (v.18.0, Exiqon, Denmark) platform.
Scan protocol Following the washing steps the slides were scanned using an Axon GenePix 4000B microarray scanner (Axon Instruments, Foster City, CA, USA) and imported into GenePix Pro 6.0 software (Axon) for grid alignment and data extraction.
Description Total RNAs were isolated using TRIzolLS (Invitrogen, Grand Island, NY, USA) and a miRNeasy mini kit (Qiagen, Shenzhen, China), which efficiently obtained all RNA species, including miRNAs. RNA was measured using the NanoDrop 1000 (ND-1000, Nanodrop Technologies), and then labeled using the miRCURY™ Hy3™/Hy5™ Power labeling kit (Exiqon, Vedbaek, Denmark) and hybridized on the specific miRCURY ™ LNA Array (v.18.0, Exiqon, Denmark) platform containing 3100 capture probes.
NC
Data processing Replicated miRNAs were averaged and miRNAs that intensities>=30 in all samples were chosen for calculating normalization factor. Expression data were normalized using the median normalization.
 
Submission date Aug 03, 2015
Last update date Aug 04, 2015
Contact name Xiangmei Kong
Phone +86-021-64377134
Organization name Eye & ENT Hospital of Fudan University
Department Department of Ophthalmology
Street address 83 Fenyang Road, Xuhui District
City Shanghai,China
ZIP/Postal code 200031
Country China
 
Platform ID GPL19128
Series (1)
GSE71639 Molecular Mechanisms Associated with Primary Open-angle Glaucoma with MicroRNA Microarray Data

Supplementary file Size Download File type/resource
GSM1842177_NC.gpr.gz 1006.9 Kb (ftp)(http) GPR
Processed data are available on Series record

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