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Status |
Public on Aug 04, 2015 |
Title |
Normal group C |
Sample type |
RNA |
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Source name |
normal group
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Organism |
Homo sapiens |
Characteristics |
subject group: patients with cataract undergoing cataract surgery mean age: 68.0 years gender (male: female) ratio in the group: 3:2 tissue: Aqueous humor
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Extracted molecule |
total RNA |
Extraction protocol |
At the beginning of the surgery the aqueous humor was collected as previously reported. After a corneal paracentesis, a 30-gauge needle on a tuberculin microsyringe was used to aspirate aqueous humor from the anterior chamber, not contacting any iris or lens tissue during the whole sample collection. Approximately 0.1-0.2ml of aqueous humor was collected from each patient, transferred to 1.5ml tubes, and immediately placed on dry ice. Then the samples were subsequently stored at −80°C until analysis.
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Label |
Hy3
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Label protocol |
Total RNAs (100 ng) were hy3-labeled using the Low Input Quick Amp Labeling Kit, one-color (Agilent) according to the manufacturer's instructions.
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Hybridization protocol |
The samples were labeled using the miRCURY™ Hy3™/Hy5™ Power labeling kit (Exiqon, Vedbaek, Denmark) and hybridized on the specific miRCURY ™ LNA Array (v.18.0, Exiqon, Denmark) platform.
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Scan protocol |
Following the washing steps the slides were scanned using an Axon GenePix 4000B microarray scanner (Axon Instruments, Foster City, CA, USA) and imported into GenePix Pro 6.0 software (Axon) for grid alignment and data extraction.
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Description |
Total RNAs were isolated using TRIzolLS (Invitrogen, Grand Island, NY, USA) and a miRNeasy mini kit (Qiagen, Shenzhen, China), which efficiently obtained all RNA species, including miRNAs. RNA was measured using the NanoDrop 1000 (ND-1000, Nanodrop Technologies), and then labeled using the miRCURY™ Hy3™/Hy5™ Power labeling kit (Exiqon, Vedbaek, Denmark) and hybridized on the specific miRCURY ™ LNA Array (v.18.0, Exiqon, Denmark) platform containing 3100 capture probes. NC
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Data processing |
Replicated miRNAs were averaged and miRNAs that intensities>=30 in all samples were chosen for calculating normalization factor. Expression data were normalized using the median normalization.
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Submission date |
Aug 03, 2015 |
Last update date |
Aug 04, 2015 |
Contact name |
Xiangmei Kong |
Phone |
+86-021-64377134
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Organization name |
Eye & ENT Hospital of Fudan University
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Department |
Department of Ophthalmology
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Street address |
83 Fenyang Road, Xuhui District
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City |
Shanghai,China |
ZIP/Postal code |
200031 |
Country |
China |
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Platform ID |
GPL19128 |
Series (1) |
GSE71639 |
Molecular Mechanisms Associated with Primary Open-angle Glaucoma with MicroRNA Microarray Data |
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Supplementary file |
Size |
Download |
File type/resource |
GSM1842177_NC.gpr.gz |
1006.9 Kb |
(ftp)(http) |
GPR |
Processed data are available on Series record |
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