|
| Status |
Public on Apr 29, 2016 |
| Title |
IL-4_72 hours, Donor 2 |
| Sample type |
RNA |
| |
|
| Source name |
peripheral blood monocyte-derived macrophages
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: peripheral blood
cell type: monocyte-derived macrophages
cell surface marker: CD14+
|
| Treatment protocol |
Human peripheral blood-derived CD14+ monocytes were exposed to IL-4 for 12 or 72 hours.
|
| Growth protocol |
CD14+ monocytes were separated from human peripheral blood and plated in 6-well cell culture plate (2 million cell/well).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Total RNA samples were labeled by using FlashTag™ Biotin HSR RNA Labeling Kit according to manufacturer's protocol.
|
| |
|
| Hybridization protocol |
Samples were hybridized at 45 degrees Celsius for 16 hours and then standard washing protocol was performed using GeneChip Fluidics Station 450 (Affymetrix).
|
| Scan protocol |
Arrays were scanned on GeneChip Scanner 7G (Affymetrix), Affymetrix GeneChip Command Console (AGCC) was used to obtain image files.
|
| Description |
16A
small RNA expression of peripheral blood monocyte-derived macrophages
|
| Data processing |
CEL files were processed by Affymetrix miRNA QCtool software. Downstream data analysis was performed in GeneSpring GX12 software.
|
| |
|
| Submission date |
Aug 03, 2015 |
| Last update date |
Apr 29, 2016 |
| Contact name |
Szilard Poliska |
| E-mail(s) |
poliska@dote.hu
|
| Phone |
+3652416432
|
| Organization name |
University of Debrecen
|
| Department |
Biochemistry and Molecular Biology
|
| Lab |
Nuclear Hormone Receptor Research Laboratory
|
| Street address |
Egyetem ter 1.
|
| City |
Debrecen |
| ZIP/Postal code |
H-4012 |
| Country |
Hungary |
| |
|
| Platform ID |
GPL8786 |
| Series (2) |
| GSE71641 |
Human periheral blood-derived monocytes and unstimulated as well as IL-4-stimulated differentiating macrophages |
| GSE71644 |
The IL4-STAT6 signaling axis establishes a conserved microRNA signature in human and mouse macrophages regulating cell survival via miR-342-3p |
|
