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Sample GSM1842203 Query DataSets for GSM1842203
Status Public on Apr 29, 2016
Title negative control mimics-transfected RAW264.7 cells replicate 1
Sample type RNA
 
Source name RAW264.7 cell line_negative_control
Organism Mus musculus
Characteristics cell line: RAW264.7
cell type: macrophage
Treatment protocol RAW264.7 mouse macrophages were transfected with negative control and miR-342-3p mimics. Total RNA was isolated 18 hours after the transfection.
Growth protocol RAW264.7 mouse macrophages were plated in 12-well cell culture plate (500000 cells/well).
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Ambion WT Expression Kit (Applied Biosystems) and GeneChip WT Terminal Labeling and Control Kit (Affymetrix) were used for amplifying and labeling 150 ng of RNA samples.
 
Hybridization protocol Samples were hybridized at 45 degrees Celsius for 16 hours and then standard washing protocol was performed using GeneChip Fluidics Station 450 (Affymetrix).
Scan protocol Arrays were scanned on GeneChip Scanner 7G (Affymetrix), Affymetrix GeneChip Command Console (AGCC) was used to obtain image files.
Description gene expression data of RAW264.7 cells transfected by negative control mimics
Data processing CHP files were generated using Affymetrix Expression Control. Downstream data analysis was performed in GeneSpring GX 12, CEL files were imported using RMA algorithm and median normalization was performed.
 
Submission date Aug 03, 2015
Last update date Apr 29, 2016
Contact name Szilard Poliska
E-mail(s) poliska@dote.hu
Phone +3652416432
Organization name University of Debrecen
Department Biochemistry and Molecular Biology
Lab Nuclear Hormone Receptor Research Laboratory
Street address Egyetem ter 1.
City Debrecen
ZIP/Postal code H-4012
Country Hungary
 
Platform ID GPL6246
Series (2)
GSE71642 Negative control and mir-342-3p mimics-transfected RAW264.7 mouse macrophages.
GSE71644 The IL4-STAT6 signaling axis establishes a conserved microRNA signature in human and mouse macrophages regulating cell survival via miR-342-3p

Data table header descriptions
ID_REF
VALUE Quantification

Data table
ID_REF VALUE
10338001 11.1167
10338002 7.34613
10338003 9.62636
10338004 8.17076
10338005 2.02874
10338006 2.49049
10338007 3.6693
10338008 5.09056
10338009 9.01769
10338010 2.04014
10338011 6.95484
10338012 2.31047
10338013 1.88809
10338014 1.90532
10338015 1.84913
10338016 8.53296
10338017 12.092
10338018 7.87211
10338019 6.44024
10338020 8.83627

Total number of rows: 35556

Table truncated, full table size 586 Kbytes.




Supplementary file Size Download File type/resource
GSM1842203_1_mir_MoGene-1_0-st-v1_.CEL.gz 4.2 Mb (ftp)(http) CEL
GSM1842203_1_mir_MoGene-1_0-st-v1_.rma-gene-default.chp.gz 270.4 Kb (ftp)(http) CHP
Raw data provided as supplementary file
Processed data included within Sample table

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