|
| Status |
Public on Apr 29, 2016 |
| Title |
negative control mimics-transfected RAW264.7 cells replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
RAW264.7 cell line_negative_control
|
| Organism |
Mus musculus |
| Characteristics |
cell line: RAW264.7
cell type: macrophage
|
| Treatment protocol |
RAW264.7 mouse macrophages were transfected with negative control and miR-342-3p mimics. Total RNA was isolated 18 hours after the transfection.
|
| Growth protocol |
RAW264.7 mouse macrophages were plated in 12-well cell culture plate (500000 cells/well).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Ambion WT Expression Kit (Applied Biosystems) and GeneChip WT Terminal Labeling and Control Kit (Affymetrix) were used for amplifying and labeling 150 ng of RNA samples.
|
| |
|
| Hybridization protocol |
Samples were hybridized at 45 degrees Celsius for 16 hours and then standard washing protocol was performed using GeneChip Fluidics Station 450 (Affymetrix).
|
| Scan protocol |
Arrays were scanned on GeneChip Scanner 7G (Affymetrix), Affymetrix GeneChip Command Console (AGCC) was used to obtain image files.
|
| Description |
gene expression data of RAW264.7 cells transfected by negative control mimics
|
| Data processing |
CHP files were generated using Affymetrix Expression Control. Downstream data analysis was performed in GeneSpring GX 12, CEL files were imported using RMA algorithm and median normalization was performed.
|
| |
|
| Submission date |
Aug 03, 2015 |
| Last update date |
Apr 29, 2016 |
| Contact name |
Szilard Poliska |
| E-mail(s) |
poliska@dote.hu
|
| Phone |
+3652416432
|
| Organization name |
University of Debrecen
|
| Department |
Biochemistry and Molecular Biology
|
| Lab |
Nuclear Hormone Receptor Research Laboratory
|
| Street address |
Egyetem ter 1.
|
| City |
Debrecen |
| ZIP/Postal code |
H-4012 |
| Country |
Hungary |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE71642 |
Negative control and mir-342-3p mimics-transfected RAW264.7 mouse macrophages. |
| GSE71644 |
The IL4-STAT6 signaling axis establishes a conserved microRNA signature in human and mouse macrophages regulating cell survival via miR-342-3p |
|
