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Sample GSM1842205 Query DataSets for GSM1842205
Status Public on Apr 29, 2016
Title negative control mimics-transfected RAW264.7 cells replicate 3
Sample type RNA
 
Source name RAW264.7 cell line_negative_control
Organism Mus musculus
Characteristics cell line: RAW264.7
cell type: macrophage
Treatment protocol RAW264.7 mouse macrophages were transfected with negative control and miR-342-3p mimics. Total RNA was isolated 18 hours after the transfection.
Growth protocol RAW264.7 mouse macrophages were plated in 12-well cell culture plate (500000 cells/well).
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Ambion WT Expression Kit (Applied Biosystems) and GeneChip WT Terminal Labeling and Control Kit (Affymetrix) were used for amplifying and labeling 150 ng of RNA samples.
 
Hybridization protocol Samples were hybridized at 45 degrees Celsius for 16 hours and then standard washing protocol was performed using GeneChip Fluidics Station 450 (Affymetrix).
Scan protocol Arrays were scanned on GeneChip Scanner 7G (Affymetrix), Affymetrix GeneChip Command Console (AGCC) was used to obtain image files.
Description gene expression data of RAW264.7 cells transfected by negative control mimics
Data processing CHP files were generated using Affymetrix Expression Control. Downstream data analysis was performed in GeneSpring GX 12, CEL files were imported using RMA algorithm and median normalization was performed.
 
Submission date Aug 03, 2015
Last update date Apr 29, 2016
Contact name Szilard Poliska
E-mail(s) poliska@dote.hu
Phone +3652416432
Organization name University of Debrecen
Department Biochemistry and Molecular Biology
Lab Nuclear Hormone Receptor Research Laboratory
Street address Egyetem ter 1.
City Debrecen
ZIP/Postal code H-4012
Country Hungary
 
Platform ID GPL6246
Series (2)
GSE71642 Negative control and mir-342-3p mimics-transfected RAW264.7 mouse macrophages.
GSE71644 The IL4-STAT6 signaling axis establishes a conserved microRNA signature in human and mouse macrophages regulating cell survival via miR-342-3p

Data table header descriptions
ID_REF
VALUE Quantification

Data table
ID_REF VALUE
10338001 11.1957
10338002 7.33082
10338003 9.67751
10338004 8.22986
10338005 1.97889
10338006 2.41648
10338007 3.54493
10338008 4.98742
10338009 9.03803
10338010 1.99537
10338011 6.9186
10338012 2.2455
10338013 1.81073
10338014 1.86907
10338015 1.79098
10338016 8.55397
10338017 12.2036
10338018 7.8581
10338019 6.4271
10338020 8.8471

Total number of rows: 35556

Table truncated, full table size 586 Kbytes.




Supplementary file Size Download File type/resource
GSM1842205_4_mir_MoGene-1_0-st-v1_.CEL.gz 4.2 Mb (ftp)(http) CEL
GSM1842205_4_mir_MoGene-1_0-st-v1_.rma-gene-default.chp.gz 270.1 Kb (ftp)(http) CHP
Raw data provided as supplementary file
Processed data included within Sample table

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