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Status |
Public on Apr 29, 2016 |
Title |
mir-342-3p mimics-transfected RAW264.7 cells replicate 2 |
Sample type |
RNA |
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Source name |
RAW264.7 cell line_mir-342-3p_mimic
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Organism |
Mus musculus |
Characteristics |
cell line: RAW264.7 cell type: macrophage
|
Treatment protocol |
RAW264.7 mouse macrophages were transfected with negative control and miR-342-3p mimics. Total RNA was isolated 18 hours after the transfection.
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Growth protocol |
RAW264.7 mouse macrophages were plated in 12-well cell culture plate (500000 cells/well).
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Ambion WT Expression Kit (Applied Biosystems) and GeneChip WT Terminal Labeling and Control Kit (Affymetrix) were used for amplifying and labeling 150 ng of RNA samples.
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Hybridization protocol |
Samples were hybridized at 45 degrees Celsius for 16 hours and then standard washing protocol was performed using GeneChip Fluidics Station 450 (Affymetrix).
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Scan protocol |
Arrays were scanned on GeneChip Scanner 7G (Affymetrix), Affymetrix GeneChip Command Console (AGCC) was used to obtain image files.
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Description |
gene expression data of RAW264.7 cells transfected by mir-342-3p mimics
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Data processing |
CHP files were generated using Affymetrix Expression Control. Downstream data analysis was performed in GeneSpring GX 12, CEL files were imported using RMA algorithm and median normalization was performed.
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Submission date |
Aug 03, 2015 |
Last update date |
Apr 29, 2016 |
Contact name |
Szilard Poliska |
E-mail(s) |
poliska@dote.hu
|
Phone |
+3652416432
|
Organization name |
University of Debrecen
|
Department |
Biochemistry and Molecular Biology
|
Lab |
Nuclear Hormone Receptor Research Laboratory
|
Street address |
Egyetem ter 1.
|
City |
Debrecen |
ZIP/Postal code |
H-4012 |
Country |
Hungary |
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Platform ID |
GPL6246 |
Series (2) |
GSE71642 |
Negative control and mir-342-3p mimics-transfected RAW264.7 mouse macrophages. |
GSE71644 |
The IL4-STAT6 signaling axis establishes a conserved microRNA signature in human and mouse macrophages regulating cell survival via miR-342-3p |
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