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Sample GSM1849546 Query DataSets for GSM1849546
Status Public on Jun 29, 2016
Title Macrophage_M2_Ctrl_rep2
Sample type RNA
 
Source name Alveolar macrophages, M2 population, derived from wildtype mice
Organism Mus musculus
Characteristics age: 8-12 weeks
strain: C57Bl/6J
cell type: Alveolar macrophage
cell population: M2
Treatment protocol As described in the paper. Briefly, primary alveolar macrophages were isolated from the lungs of mice by bronchoalveolar lavage (BAL) with 8 washes of 1 ml PBS at room temperature. Polarization towards the M2 phenotype was done with IL-4 (20 ng/ml, Immunotool) treatment for up to 24 h. Unpolarized cells (M0) served as controls.
Growth protocol Animals housed under spf conditions
Extracted molecule total RNA
Extraction protocol RNeasy Mini Kit (Qiagen) including DNase treatment
Label Biotin
Label protocol Ovation PicoSL WTA System V2 in combination with the Encore Biotin IL Module (Nugen)
 
Hybridization protocol Standard Illumina hybridization protocol including minor modification as suggested by Nugen
Scan protocol Standard Illumina scanning protocol (HiScan)
Description Gene expression data from alveolar macrophages, M2 population, derived from wildtype mice
Data processing Data including background subtraction (GenomeStudioV2011.1 software, gene expression module version 1.9.0), removal of negative values by introducing an offset, quantile normalisation
 
Submission date Aug 12, 2015
Last update date Jun 29, 2016
Contact name Johannes Beckers
E-mail(s) johannes.beckers@helmholtz-munich.de
Organization name Helmholtz Zentrum Muenchen
Department Institute of Experimental Genetics
Street address Ingolstaedter Landstr. 1
City Neuherberg
ZIP/Postal code 85764
Country Germany
 
Platform ID GPL6885
Series (1)
GSE72000 Immunoproteasome dysfunction augments alternative polarization of alveolar macrophages

Data table header descriptions
ID_REF
VALUE Linear expression values
WT_M2_08.Detection Pval

Data table
ID_REF VALUE WT_M2_08.Detection Pval
ILMN_1212607 92 2.13E-01
ILMN_1212612 43 9.21E-01
ILMN_1212619 84 2.74E-01
ILMN_1212628 59 6.62E-01
ILMN_1212632 94 1.92E-01
ILMN_1212636 652 0.00E+00
ILMN_1212637 302 0.00E+00
ILMN_1212645 56 7.14E-01
ILMN_1212648 88 2.41E-01
ILMN_1212653 56 7.23E-01
ILMN_1212672 152 1.13E-02
ILMN_1212682 118 7.02E-02
ILMN_1212683 74 3.87E-01
ILMN_1212685 277 0.00E+00
ILMN_1212692 50 8.11E-01
ILMN_1212693 52 7.82E-01
ILMN_1212695 80 3.16E-01
ILMN_1212698 109 1.09E-01
ILMN_1212702 59 6.62E-01
ILMN_1212703 261 0.00E+00

Total number of rows: 25697

Table truncated, full table size 638 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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