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Status |
Public on Jun 29, 2016 |
Title |
Macrophage_M2_Ctrl_rep2 |
Sample type |
RNA |
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Source name |
Alveolar macrophages, M2 population, derived from wildtype mice
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Organism |
Mus musculus |
Characteristics |
age: 8-12 weeks strain: C57Bl/6J cell type: Alveolar macrophage cell population: M2
|
Treatment protocol |
As described in the paper. Briefly, primary alveolar macrophages were isolated from the lungs of mice by bronchoalveolar lavage (BAL) with 8 washes of 1 ml PBS at room temperature. Polarization towards the M2 phenotype was done with IL-4 (20 ng/ml, Immunotool) treatment for up to 24 h. Unpolarized cells (M0) served as controls.
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Growth protocol |
Animals housed under spf conditions
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Extracted molecule |
total RNA |
Extraction protocol |
RNeasy Mini Kit (Qiagen) including DNase treatment
|
Label |
Biotin
|
Label protocol |
Ovation PicoSL WTA System V2 in combination with the Encore Biotin IL Module (Nugen)
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|
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Hybridization protocol |
Standard Illumina hybridization protocol including minor modification as suggested by Nugen
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Scan protocol |
Standard Illumina scanning protocol (HiScan)
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Description |
Gene expression data from alveolar macrophages, M2 population, derived from wildtype mice
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Data processing |
Data including background subtraction (GenomeStudioV2011.1 software, gene expression module version 1.9.0), removal of negative values by introducing an offset, quantile normalisation
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|
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Submission date |
Aug 12, 2015 |
Last update date |
Jun 29, 2016 |
Contact name |
Johannes Beckers |
E-mail(s) |
johannes.beckers@helmholtz-munich.de
|
Organization name |
Helmholtz Zentrum Muenchen
|
Department |
Institute of Experimental Genetics
|
Street address |
Ingolstaedter Landstr. 1
|
City |
Neuherberg |
ZIP/Postal code |
85764 |
Country |
Germany |
|
|
Platform ID |
GPL6885 |
Series (1) |
GSE72000 |
Immunoproteasome dysfunction augments alternative polarization of alveolar macrophages |
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